Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease
<i>Clostridium perfringens</i> infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (<i>Cb</i>Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm o...
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2025-01-01
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| author | Wenjing Zhang Zhenzhen Hao Daoxin Yang Wangli Ji Kairui Guo Xianhua Sun Shuai Wang Shuyan Yang Jianshuang Ma Tong Wang Huiying Luo Bin Yao Meiling Zhang Yuan Wang Huoqing Huang Xiaoyun Su |
| author_facet | Wenjing Zhang Zhenzhen Hao Daoxin Yang Wangli Ji Kairui Guo Xianhua Sun Shuai Wang Shuyan Yang Jianshuang Ma Tong Wang Huiying Luo Bin Yao Meiling Zhang Yuan Wang Huoqing Huang Xiaoyun Su |
| author_sort | Wenjing Zhang |
| collection | DOAJ |
| description | <i>Clostridium perfringens</i> infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (<i>Cb</i>Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of <i>C. perfringens,</i> were analyzed for their ability to alleviate <i>C. perfringens</i>-induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no <i>C. perfringens</i> challenge; Cp, no enzyme, <i>C. perfringens</i> challenge; Xyn, <i>Cb</i>Xyn10C plus <i>C. perfringens</i> challenge; Xyn+Am, <i>Cb</i>Xyn10C+Amylase plus <i>C. perfringens</i> challenge; Xyn+Ap, <i>Cb</i>Xyn10C+Alkaline protease plus <i>C. perfringens</i> challenge). The <i>C. perfringens</i> CVCC 60102 was administered orally on a daily basis to the chickens from 14 to 20 days. In comparison with Cp, Xyn+Ap significantly reduced intestinal damage in the duodenum, jejunum, and ileum of chickens challenged with <i>C. perfringens</i> (<i>p</i> < 0.05). The enzymes, and particularly Xyn+Ap, notably enhanced the expression of key intestinal barrier genes, reduced the IL-6 level, and decreased the DAO (diamine oxidase) level. Not unexpectedly, feeding enzymes influenced the abundance of <i>Lactobacillus</i> and <i>Butyricicoccus</i> bacteria in the intestine. These results indicated that <i>Cb</i>Xyn10C and protease can be used to alleviate intestinal damage caused by <i>C. perfringens</i> infection. |
| format | Article |
| id | doaj-art-63a0785fd5b54145ab85bdea84d999b9 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-63a0785fd5b54145ab85bdea84d999b92025-01-24T13:17:39ZengMDPI AGAnimals2076-26152025-01-0115212310.3390/ani15020123Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a ProteaseWenjing Zhang0Zhenzhen Hao1Daoxin Yang2Wangli Ji3Kairui Guo4Xianhua Sun5Shuai Wang6Shuyan Yang7Jianshuang Ma8Tong Wang9Huiying Luo10Bin Yao11Meiling Zhang12Yuan Wang13Huoqing Huang14Xiaoyun Su15State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLaboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, Shanghai 200241, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLaboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, Shanghai 200241, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China<i>Clostridium perfringens</i> infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (<i>Cb</i>Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of <i>C. perfringens,</i> were analyzed for their ability to alleviate <i>C. perfringens</i>-induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no <i>C. perfringens</i> challenge; Cp, no enzyme, <i>C. perfringens</i> challenge; Xyn, <i>Cb</i>Xyn10C plus <i>C. perfringens</i> challenge; Xyn+Am, <i>Cb</i>Xyn10C+Amylase plus <i>C. perfringens</i> challenge; Xyn+Ap, <i>Cb</i>Xyn10C+Alkaline protease plus <i>C. perfringens</i> challenge). The <i>C. perfringens</i> CVCC 60102 was administered orally on a daily basis to the chickens from 14 to 20 days. In comparison with Cp, Xyn+Ap significantly reduced intestinal damage in the duodenum, jejunum, and ileum of chickens challenged with <i>C. perfringens</i> (<i>p</i> < 0.05). The enzymes, and particularly Xyn+Ap, notably enhanced the expression of key intestinal barrier genes, reduced the IL-6 level, and decreased the DAO (diamine oxidase) level. Not unexpectedly, feeding enzymes influenced the abundance of <i>Lactobacillus</i> and <i>Butyricicoccus</i> bacteria in the intestine. These results indicated that <i>Cb</i>Xyn10C and protease can be used to alleviate intestinal damage caused by <i>C. perfringens</i> infection.https://www.mdpi.com/2076-2615/15/2/123necrotic enteritis<i>Clostridium perfringens</i>xylanaseproteasebroiler chickensgut microbiota modulation |
| spellingShingle | Wenjing Zhang Zhenzhen Hao Daoxin Yang Wangli Ji Kairui Guo Xianhua Sun Shuai Wang Shuyan Yang Jianshuang Ma Tong Wang Huiying Luo Bin Yao Meiling Zhang Yuan Wang Huoqing Huang Xiaoyun Su Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease Animals necrotic enteritis <i>Clostridium perfringens</i> xylanase protease broiler chickens gut microbiota modulation |
| title | Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease |
| title_full | Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease |
| title_fullStr | Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease |
| title_full_unstemmed | Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease |
| title_short | Alleviating <i>Clostridium perfringens</i>-Induced Intestinal Lesions in Chickens Using the Xylanase <i>Cb</i>Xyn10C and Its Binary Cocktail with a Protease |
| title_sort | alleviating i clostridium perfringens i induced intestinal lesions in chickens using the xylanase i cb i xyn10c and its binary cocktail with a protease |
| topic | necrotic enteritis <i>Clostridium perfringens</i> xylanase protease broiler chickens gut microbiota modulation |
| url | https://www.mdpi.com/2076-2615/15/2/123 |
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