High-throughput site-directed mutagenesis using oligonucleotides synthesized on DNA chips

Site-directed mutagenesis has greatly helped researchers both to understand the precise role of specific residues in coding sequences and to generate variants of proteins that have acquired new characteristics. Today's demands for more complete functional cartographies of proteins and advances...

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Bibliographic Details
Main Authors: Didier Saboulard, Vincent Dugas, Mehdi Jaber, Jérôme Broutin, Eliane Souteyrand, Julien Sylvestre, Marc Delcourt
Format: Article
Language:English
Published: Taylor & Francis Group 2005-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/05393ST04
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