Ultrasensitive detection of clinical pathogens through a target-amplification-free collateral-cleavage-enhancing CRISPR-CasΦ tool

Ultrasensitive detection of clinical pathogens through a target-amplification-free collateral-cleavage-enhancing CRISPR-CasΦ tool

Abstract Clinical pathogen diagnostics detect targets by qPCR (but with low sensitivity) or blood culturing (but time-consuming). Here we leverage a dual-stem-loop DNA amplifier to enhance non-specific collateral enzymatic cleavage of an oligonucleotide linker between a fluophore and its quencher by...

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Main Authors: Huiyou Chen, Fengge Song, Buhua Wang, Hui Huang, Yanchi Luo, Xiaosheng Han, Hewen He, Shaolu Lin, Liudang Wan, Zhengliang Huang, Zhaoyong Fu, Rodrigo Ledesma-Amaro, Dapeng Yin, Haimei Mao, Linwen He, Tao Yang, Zijing Chen, Yubin Ma, Evelyn Y. Xue, Yi Wan, Chuanbin Mao
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59219-x
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https://doi.org/10.1038/s41467-025-59219-x

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