Western blotting of native proteins from agarose gels

Western blotting of native proteins from agarose gels

We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provided native structures for a variety of proteins and...

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Bibliographic Details
Main Authors: Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta
Format: Article
Language:English
Published: Taylor & Francis Group 2022-05-01
Series:BioTechniques
Subjects:
agarose
conformation specific monoclonal antibody
GFP
native gel electrophoresis
PLXDC2
SARS-CoV-2 spike protein
Online Access:https://www.future-science.com/doi/10.2144/btn-2022-0012
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https://www.future-science.com/doi/10.2144/btn-2022-0012

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