Selection and Validation of Stable Reference Genes for RT-qPCR in <i>Scotogramma trifolii</i> (Lepidoptera: Noctuidae)

Selection and Validation of Stable Reference Genes for RT-qPCR in <i>Scotogramma trifolii</i> (Lepidoptera: Noctuidae)

The clover cutworm, <i>Scotogramma trifolii</i> Rottemberg (Lepidoptera: Noctuidae), is a globally distributed polyphagous pest causing significant economic losses to agricultural crops. RT-qPCR is a gold-standard technique for gene expression analysis, yet its accuracy depends criticall...

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Bibliographic Details
Main Authors: Anpei Yang, Hang Zhang, Weiwei Bai, Ruifeng Ding, Weipeng Li, Guangkuo Li
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Insects
Subjects:
<i>Scotogramma trifolii</i>
reference genes
gene expression
normalization
RT-qPCR
Online Access:https://www.mdpi.com/2075-4450/16/5/527
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