Doubling multiplexed imaging capability via spatial expression pattern-guided protein pairing and computational unmixing
Abstract Three-dimensional multiplexed fluorescence imaging is an indispensable technique in neuroscience. For two-dimensional multiplexed imaging, cyclic immunofluorescence, which involves repeating staining, imaging, and signal removal over multiple cycles, has been widely used. However, the appli...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-06-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08357-5 |
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