Differential Display Probes for cDNA Arrays
PCR with a combination of one arbitrary and one oligo(dT) anchor primer can be used to generate an effective probe for cDNA arrays. The method uses less than 1/200 of the amount of RNA used in some other array hybridization methods. Each fingerprint detects approximately 5% of the transcribed mRNAs,...
Saved in:
| Main Authors: | Thomas Trenkle, John Welsh, Michael McClelland |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-09-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/99273rr03 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Phage display of cDNA libraries: enrichment of cDNA expression using open reading frame selection
by: Peggy Ho Faix, et al.
Published: (2004-06-01) -
Preparation of Phage Display cDNA Libraries for Identifying Immunogenic Tumor Antigens: Challenges in Functional cDNA Presentation and Approaches to Overcoming Them
by: Nuša Brišar, et al.
Published: (2024-11-01) -
3′-End cDNA Pool Suitable for Differential Display from a Small Number of Cells
by: S. Zhao, et al.
Published: (1998-05-01) -
cDNA array monitoring of defense and signal pathway related genes in Oryza sativa
by: PEI Yan-xi, et al.
Published: (2003-07-01) -
High-Stringency Subtraction for the Identification of Differentially Regulated cDNA Clones
by: Charles P. Scutt, et al.
Published: (1997-09-01)