Differential Display Probes for cDNA Arrays

PCR with a combination of one arbitrary and one oligo(dT) anchor primer can be used to generate an effective probe for cDNA arrays. The method uses less than 1/200 of the amount of RNA used in some other array hybridization methods. Each fingerprint detects approximately 5% of the transcribed mRNAs,...

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Bibliographic Details
Main Authors: Thomas Trenkle, John Welsh, Michael McClelland
Format: Article
Language:English
Published: Taylor & Francis Group 1999-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/99273rr03
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