A novel approach to double-strand DNA break analysis through γ-H2AX confocal image quantification and bio-dosimetry

A novel approach to double-strand DNA break analysis through γ-H2AX confocal image quantification and bio-dosimetry

Abstract DNA damage occurs in all living cells. γ-H2AX imaging by fluorescent microscopy is widely used across disciplines in the analysis of double-strand break (DSB) DNA damage. Here we demonstrate a method for the quantitative analysis of such DBSs. Ionising radiation, well known to induce DSBs,...

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Bibliographic Details
Main Authors: Michael Valceski, Elette Engels, Sarah Vogel, Jason Paino, Dylan Potter, Carolyn Hollis, Abass Khochaiche, Micah Barnes, Matthew Cameron, Alice O’Keefe, Kiarn Roughley, Anatoly Rosenfeld, Michael Lerch, Stéphanie Corde, Moeava Tehei
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Scientific Reports
Subjects:
Confocal microscopy
Image analysis
Radiotherapy
Synchrotron
Microbeam Radiation Therapy
γH2AX
Online Access:https://doi.org/10.1038/s41598-024-76683-5
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https://doi.org/10.1038/s41598-024-76683-5

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