Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis
Resveratrol (trans-3,5,4′-trihydroxystilbene) (RSV) is a natural polyphenol with protective effects over cardiac tissues and can affect cell survival and differentiation in cardiac stem cells transplantation. However, whether this agent can affect cardiomyocytes (CMs) differentiation of induced plur...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/2524092 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558529838317568 |
|---|---|
| author | Hui Liu Shaoli Zhang Lihua Zhao Yan Zhang Qiuping Li Xiaoyan Chai Yongchun Zhang |
| author_facet | Hui Liu Shaoli Zhang Lihua Zhao Yan Zhang Qiuping Li Xiaoyan Chai Yongchun Zhang |
| author_sort | Hui Liu |
| collection | DOAJ |
| description | Resveratrol (trans-3,5,4′-trihydroxystilbene) (RSV) is a natural polyphenol with protective effects over cardiac tissues and can affect cell survival and differentiation in cardiac stem cells transplantation. However, whether this agent can affect cardiomyocytes (CMs) differentiation of induced pluripotent stem cells (iPSCs) is not yet clear. This study explored whether RSV can affect CMs differentiation of human iPSCs. Under embryoid bodies (EBs) condition, the effect of RSV on the change of pluripotent markers, endoderm markers, mesoderm markers, and ectoderm markers was measured using qRT-PCR. Under CM differentiation culture, the effect of RSV on CM specific markers was also measured. The regulative role of RSV over canonical Wnt signal pathway and serum response factor- (SRF-) miR-1 axis and the functions of these two axes were further studied. Results showed that RSV had no effect on the self-renewal of human iPSCs but could promote mesoderm differentiation. Under CM differentiation culture, RSV could promote CM differentiation of human iPSCs through suppressing canonical Wnt signal pathway and enhancing SRF-miR-1 axis. |
| format | Article |
| id | doaj-art-5f19a59ae88042bdbbeb3b40fb064f6d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5f19a59ae88042bdbbeb3b40fb064f6d2025-02-03T01:32:07ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/25240922524092Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 AxisHui Liu0Shaoli Zhang1Lihua Zhao2Yan Zhang3Qiuping Li4Xiaoyan Chai5Yongchun Zhang6 Department of Internal Medicine-Cardiovascular, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China Department of Internal Medicine-Cardiovascular, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China Department of Internal Medicine-Cardiovascular, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China Department of Internal Medicine-Cardiovascular, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China Department of Internal Medicine-Cardiovascular, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China Department of Internal Medicine-Cardiovascular, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, China Department of Internal Medicine-Cardiovascular, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, ChinaResveratrol (trans-3,5,4′-trihydroxystilbene) (RSV) is a natural polyphenol with protective effects over cardiac tissues and can affect cell survival and differentiation in cardiac stem cells transplantation. However, whether this agent can affect cardiomyocytes (CMs) differentiation of induced pluripotent stem cells (iPSCs) is not yet clear. This study explored whether RSV can affect CMs differentiation of human iPSCs. Under embryoid bodies (EBs) condition, the effect of RSV on the change of pluripotent markers, endoderm markers, mesoderm markers, and ectoderm markers was measured using qRT-PCR. Under CM differentiation culture, the effect of RSV on CM specific markers was also measured. The regulative role of RSV over canonical Wnt signal pathway and serum response factor- (SRF-) miR-1 axis and the functions of these two axes were further studied. Results showed that RSV had no effect on the self-renewal of human iPSCs but could promote mesoderm differentiation. Under CM differentiation culture, RSV could promote CM differentiation of human iPSCs through suppressing canonical Wnt signal pathway and enhancing SRF-miR-1 axis.http://dx.doi.org/10.1155/2016/2524092 |
| spellingShingle | Hui Liu Shaoli Zhang Lihua Zhao Yan Zhang Qiuping Li Xiaoyan Chai Yongchun Zhang Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis Stem Cells International |
| title | Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis |
| title_full | Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis |
| title_fullStr | Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis |
| title_full_unstemmed | Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis |
| title_short | Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis |
| title_sort | resveratrol enhances cardiomyocyte differentiation of human induced pluripotent stem cells through inhibiting canonical wnt signal pathway and enhancing serum response factor mir 1 axis |
| url | http://dx.doi.org/10.1155/2016/2524092 |
| work_keys_str_mv | AT huiliu resveratrolenhancescardiomyocytedifferentiationofhumaninducedpluripotentstemcellsthroughinhibitingcanonicalwntsignalpathwayandenhancingserumresponsefactormir1axis AT shaolizhang resveratrolenhancescardiomyocytedifferentiationofhumaninducedpluripotentstemcellsthroughinhibitingcanonicalwntsignalpathwayandenhancingserumresponsefactormir1axis AT lihuazhao resveratrolenhancescardiomyocytedifferentiationofhumaninducedpluripotentstemcellsthroughinhibitingcanonicalwntsignalpathwayandenhancingserumresponsefactormir1axis AT yanzhang resveratrolenhancescardiomyocytedifferentiationofhumaninducedpluripotentstemcellsthroughinhibitingcanonicalwntsignalpathwayandenhancingserumresponsefactormir1axis AT qiupingli resveratrolenhancescardiomyocytedifferentiationofhumaninducedpluripotentstemcellsthroughinhibitingcanonicalwntsignalpathwayandenhancingserumresponsefactormir1axis AT xiaoyanchai resveratrolenhancescardiomyocytedifferentiationofhumaninducedpluripotentstemcellsthroughinhibitingcanonicalwntsignalpathwayandenhancingserumresponsefactormir1axis AT yongchunzhang resveratrolenhancescardiomyocytedifferentiationofhumaninducedpluripotentstemcellsthroughinhibitingcanonicalwntsignalpathwayandenhancingserumresponsefactormir1axis |