Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis
Poly-γ-glutamic acid (γ-PGA), naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease (IBD), inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PG...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/982383 |
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| author | Munkhtugs Davaatseren Jin-Taek Hwang Jae Ho Park Myung-Sunny Kim Shuaiyu Wang Mi Jeong Sung |
| author_facet | Munkhtugs Davaatseren Jin-Taek Hwang Jae Ho Park Myung-Sunny Kim Shuaiyu Wang Mi Jeong Sung |
| author_sort | Munkhtugs Davaatseren |
| collection | DOAJ |
| description | Poly-γ-glutamic acid (γ-PGA), naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease (IBD), inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PGA effects on angiogenesis and inflammation in a dextran sulfate sodium- (DSS-) induced mouse colitis model. Experimental colitis was induced in male C57BL/6 mice by administering 3% DSS. Disease activity index (DAI), histopathological scores, microvascular density, myeloperoxidase activity, and VEGF-A and VEGFR2 expression were compared among control mice, DSS-treated mice, and mice receiving 3% DSS along with γ-PGA at 50 mg/kg body weight per day or 3% DSS with γ-PGA at 200 mg/kg body weight per day. We found that γ-PGA significantly attenuated weight loss, DAI, and colon shortening. γ-PGA also significantly reduced histopathological evidence of injury. Moreover, γ-PGA significantly attenuated DSS-induced blood vessel densities. Furthermore, γ-PGA attenuated DSS-induced expression of VEGF-A and its receptor, VEGFR2. In addition, γ-PGA treatment led to reduced recruitment of leukocytes to the inflamed colon. Therefore, our results indicate that γ-PGA has potential application in conditions marked by inflammatory-driven angiogenesis and mucosal inflammation. |
| format | Article |
| id | doaj-art-5e9c414b31c84262adb54c3f95fd5dec |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5e9c414b31c84262adb54c3f95fd5dec2025-02-03T06:01:34ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/982383982383Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental ColitisMunkhtugs Davaatseren0Jin-Taek Hwang1Jae Ho Park2Myung-Sunny Kim3Shuaiyu Wang4Mi Jeong Sung5Research Division Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Ku, Seongnam Gyeonggi 463-746, Republic of KoreaResearch Division Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Ku, Seongnam Gyeonggi 463-746, Republic of KoreaResearch Division Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Ku, Seongnam Gyeonggi 463-746, Republic of KoreaResearch Division Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Ku, Seongnam Gyeonggi 463-746, Republic of KoreaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road Qingdao, Shandong 266071, ChinaResearch Division Emerging Innovative Technology, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Ku, Seongnam Gyeonggi 463-746, Republic of KoreaPoly-γ-glutamic acid (γ-PGA), naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease (IBD), inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PGA effects on angiogenesis and inflammation in a dextran sulfate sodium- (DSS-) induced mouse colitis model. Experimental colitis was induced in male C57BL/6 mice by administering 3% DSS. Disease activity index (DAI), histopathological scores, microvascular density, myeloperoxidase activity, and VEGF-A and VEGFR2 expression were compared among control mice, DSS-treated mice, and mice receiving 3% DSS along with γ-PGA at 50 mg/kg body weight per day or 3% DSS with γ-PGA at 200 mg/kg body weight per day. We found that γ-PGA significantly attenuated weight loss, DAI, and colon shortening. γ-PGA also significantly reduced histopathological evidence of injury. Moreover, γ-PGA significantly attenuated DSS-induced blood vessel densities. Furthermore, γ-PGA attenuated DSS-induced expression of VEGF-A and its receptor, VEGFR2. In addition, γ-PGA treatment led to reduced recruitment of leukocytes to the inflamed colon. Therefore, our results indicate that γ-PGA has potential application in conditions marked by inflammatory-driven angiogenesis and mucosal inflammation.http://dx.doi.org/10.1155/2013/982383 |
| spellingShingle | Munkhtugs Davaatseren Jin-Taek Hwang Jae Ho Park Myung-Sunny Kim Shuaiyu Wang Mi Jeong Sung Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis Mediators of Inflammation |
| title | Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_full | Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_fullStr | Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_full_unstemmed | Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_short | Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_sort | poly γ glutamic acid attenuates angiogenesis and inflammation in experimental colitis |
| url | http://dx.doi.org/10.1155/2013/982383 |
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