Dictyostelium discoideum transformation by oscillating electric field electroporation
Dictyostelium discoideum has been used as a genetically tractable model organism to study many biological phenomena. High-efficiency transformation is a prerequisite for successful genetic screens such as mutant complementation, identification of suppressor genes, or insertional mutagenesis. Althoug...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2003-07-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03351st03 |
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| _version_ | 1850152541264805888 |
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| author | Laeticia Alibaud Pierre Cosson Mohammed Benghezal |
| author_facet | Laeticia Alibaud Pierre Cosson Mohammed Benghezal |
| author_sort | Laeticia Alibaud |
| collection | DOAJ |
| description | Dictyostelium discoideum has been used as a genetically tractable model organism to study many biological phenomena. High-efficiency transformation is a prerequisite for successful genetic screens such as mutant complementation, identification of suppressor genes, or insertional mutagenesis. Although exponential decay electroporation is the standard transformation technique for D. discoideum, its efficiency is relatively low and its reproducibility is weak. Here we optimized the oscillating electroporation technique for D. discoideum transformation and compared it to the exponential decay electroporation. A 20-fold increase in the efficiency was reproducibly achieved. This alternative electroporation technique should facilitate future genetic approaches in D. discoideum. |
| format | Article |
| id | doaj-art-5db0c57c446c43cd978f6544aa54e91a |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2003-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-5db0c57c446c43cd978f6544aa54e91a2025-08-20T02:25:57ZengTaylor & Francis GroupBioTechniques0736-62051940-98182003-07-01351788310.2144/03351st03Dictyostelium discoideum transformation by oscillating electric field electroporationLaeticia Alibaud0Pierre Cosson1Mohammed Benghezal21Université de Genève, Switzerland1Université de Genève, Switzerland2Athelas SA, Geneva, SwitzerlandDictyostelium discoideum has been used as a genetically tractable model organism to study many biological phenomena. High-efficiency transformation is a prerequisite for successful genetic screens such as mutant complementation, identification of suppressor genes, or insertional mutagenesis. Although exponential decay electroporation is the standard transformation technique for D. discoideum, its efficiency is relatively low and its reproducibility is weak. Here we optimized the oscillating electroporation technique for D. discoideum transformation and compared it to the exponential decay electroporation. A 20-fold increase in the efficiency was reproducibly achieved. This alternative electroporation technique should facilitate future genetic approaches in D. discoideum.https://www.future-science.com/doi/10.2144/03351st03 |
| spellingShingle | Laeticia Alibaud Pierre Cosson Mohammed Benghezal Dictyostelium discoideum transformation by oscillating electric field electroporation BioTechniques |
| title | Dictyostelium discoideum transformation by oscillating electric field electroporation |
| title_full | Dictyostelium discoideum transformation by oscillating electric field electroporation |
| title_fullStr | Dictyostelium discoideum transformation by oscillating electric field electroporation |
| title_full_unstemmed | Dictyostelium discoideum transformation by oscillating electric field electroporation |
| title_short | Dictyostelium discoideum transformation by oscillating electric field electroporation |
| title_sort | dictyostelium discoideum transformation by oscillating electric field electroporation |
| url | https://www.future-science.com/doi/10.2144/03351st03 |
| work_keys_str_mv | AT laeticiaalibaud dictyosteliumdiscoideumtransformationbyoscillatingelectricfieldelectroporation AT pierrecosson dictyosteliumdiscoideumtransformationbyoscillatingelectricfieldelectroporation AT mohammedbenghezal dictyosteliumdiscoideumtransformationbyoscillatingelectricfieldelectroporation |