Dictyostelium discoideum transformation by oscillating electric field electroporation

Dictyostelium discoideum has been used as a genetically tractable model organism to study many biological phenomena. High-efficiency transformation is a prerequisite for successful genetic screens such as mutant complementation, identification of suppressor genes, or insertional mutagenesis. Althoug...

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Bibliographic Details
Main Authors: Laeticia Alibaud, Pierre Cosson, Mohammed Benghezal
Format: Article
Language:English
Published: Taylor & Francis Group 2003-07-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/03351st03
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