Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceus
Objective: To optimize the preparation method of Hericium erinaceus-derived extracellular nanovesicles and investigate their antioxidant activity and protective effect against oxidative damage in Caco-2 cells. Methods: Hericium erinaceus-derived extracellular nanovesicles (HEDENVs) were isolated fro...
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The editorial department of Science and Technology of Food Industry
2025-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030095 |
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| author | Xueli YANG Ben NIU Hangjun CHEN Weijie WU Guannan WANG Xiangjun FANG Honglei MU Haiyan GAO |
| author_facet | Xueli YANG Ben NIU Hangjun CHEN Weijie WU Guannan WANG Xiangjun FANG Honglei MU Haiyan GAO |
| author_sort | Xueli YANG |
| collection | DOAJ |
| description | Objective: To optimize the preparation method of Hericium erinaceus-derived extracellular nanovesicles and investigate their antioxidant activity and protective effect against oxidative damage in Caco-2 cells. Methods: Hericium erinaceus-derived extracellular nanovesicles (HEDENVs) were isolated from fresh Hericium erinaceus by ultracentrifugation and sucrose gradient ultracentrifugation. The optimal extraction process of HEDENVs was optimized by one-way test and response surface test, while the particle size, Zeta potential, and morphology of HEDENVs were characterized by a laser particle size instrument and transmission electron microscope. Lipidomics and proteomics analysis of HEDENVs was performed by liquid chromatography-mass spectrometry. The in vitro antioxidant activity of HEDENVs was evaluated by determining the DPPH, ABTS+, and O2− radical scavenging capacity. A Caco-2 cell oxidative stress damage model was created using H2O2, and changes in ROS content in cells were measured using laser confocal microscopy. Results: The optimal extraction process of HEDENVs was the 25% interface of the sucrose concentration, centrifugation time was 2 h, and centrifugal speed was 86000 r/min, at which the concentration of HEDENVs was 402.24 μg/mL. HEDENVs were nanovesicles with a lipid bilayer membrane, the particle size was 110.7±16.9 nm, PDI=0.551, and the Zeta potential was −14.2 mV. Based on the analysis of lipidomics and proteomics, 346 lipids species and 52 proteins were identified in HEDENVs. The results of in vitro antioxidant test showed that the scavenging rate of 400 μg/mL HEDENVs for DPPH free radical was 94.79%, the scavenging rate for ABTS+ free radical was 96.67%, and the scavenging rate for O2− free radical was 96.83%. An oxidative damage model in Caco-2 cells was established by H2O2, HEDENVs could significantly reduce the production of ROS in damaged cells. Conclusion: It showed that HEDENVs had certain antioxidant activity in various antioxidant experiment. |
| format | Article |
| id | doaj-art-5d7d1e96a68a4286898ab721c0de7b3e |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-5d7d1e96a68a4286898ab721c0de7b3e2025-01-10T06:49:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-01-0146221823010.13386/j.issn1002-0306.20240300952024030095-2Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceusXueli YANG0Ben NIU1Hangjun CHEN2Weijie WU3Guannan WANG4Xiangjun FANG5Honglei MU6Haiyan GAO7College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Fruit Postharvest Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruit and Vegetable Preservation and Processing Technology of Zhejiang Province, Key Laboratory of Fruit and Vegetable Preservation and Processing of China Light Industry, Hangzhou 310021, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Fruit Postharvest Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruit and Vegetable Preservation and Processing Technology of Zhejiang Province, Key Laboratory of Fruit and Vegetable Preservation and Processing of China Light Industry, Hangzhou 310021, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Fruit Postharvest Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruit and Vegetable Preservation and Processing Technology of Zhejiang Province, Key Laboratory of Fruit and Vegetable Preservation and Processing of China Light Industry, Hangzhou 310021, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Fruit Postharvest Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruit and Vegetable Preservation and Processing Technology of Zhejiang Province, Key Laboratory of Fruit and Vegetable Preservation and Processing of China Light Industry, Hangzhou 310021, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Fruit Postharvest Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruit and Vegetable Preservation and Processing Technology of Zhejiang Province, Key Laboratory of Fruit and Vegetable Preservation and Processing of China Light Industry, Hangzhou 310021, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Fruit Postharvest Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruit and Vegetable Preservation and Processing Technology of Zhejiang Province, Key Laboratory of Fruit and Vegetable Preservation and Processing of China Light Industry, Hangzhou 310021, ChinaInstitute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Fruit Postharvest Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruit and Vegetable Preservation and Processing Technology of Zhejiang Province, Key Laboratory of Fruit and Vegetable Preservation and Processing of China Light Industry, Hangzhou 310021, ChinaObjective: To optimize the preparation method of Hericium erinaceus-derived extracellular nanovesicles and investigate their antioxidant activity and protective effect against oxidative damage in Caco-2 cells. Methods: Hericium erinaceus-derived extracellular nanovesicles (HEDENVs) were isolated from fresh Hericium erinaceus by ultracentrifugation and sucrose gradient ultracentrifugation. The optimal extraction process of HEDENVs was optimized by one-way test and response surface test, while the particle size, Zeta potential, and morphology of HEDENVs were characterized by a laser particle size instrument and transmission electron microscope. Lipidomics and proteomics analysis of HEDENVs was performed by liquid chromatography-mass spectrometry. The in vitro antioxidant activity of HEDENVs was evaluated by determining the DPPH, ABTS+, and O2− radical scavenging capacity. A Caco-2 cell oxidative stress damage model was created using H2O2, and changes in ROS content in cells were measured using laser confocal microscopy. Results: The optimal extraction process of HEDENVs was the 25% interface of the sucrose concentration, centrifugation time was 2 h, and centrifugal speed was 86000 r/min, at which the concentration of HEDENVs was 402.24 μg/mL. HEDENVs were nanovesicles with a lipid bilayer membrane, the particle size was 110.7±16.9 nm, PDI=0.551, and the Zeta potential was −14.2 mV. Based on the analysis of lipidomics and proteomics, 346 lipids species and 52 proteins were identified in HEDENVs. The results of in vitro antioxidant test showed that the scavenging rate of 400 μg/mL HEDENVs for DPPH free radical was 94.79%, the scavenging rate for ABTS+ free radical was 96.67%, and the scavenging rate for O2− free radical was 96.83%. An oxidative damage model in Caco-2 cells was established by H2O2, HEDENVs could significantly reduce the production of ROS in damaged cells. Conclusion: It showed that HEDENVs had certain antioxidant activity in various antioxidant experiment.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030095hericium erinaceusnanovesiclesprocess optimizationstructural characterizationomics analysiscaco-2 cellsantioxidation |
| spellingShingle | Xueli YANG Ben NIU Hangjun CHEN Weijie WU Guannan WANG Xiangjun FANG Honglei MU Haiyan GAO Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceus Shipin gongye ke-ji hericium erinaceus nanovesicles process optimization structural characterization omics analysis caco-2 cells antioxidation |
| title | Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceus |
| title_full | Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceus |
| title_fullStr | Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceus |
| title_full_unstemmed | Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceus |
| title_short | Preparation, Structural Characterization and Antioxidant Activity of Extracellular Nanovesicles from Hericium erinaceus |
| title_sort | preparation structural characterization and antioxidant activity of extracellular nanovesicles from hericium erinaceus |
| topic | hericium erinaceus nanovesicles process optimization structural characterization omics analysis caco-2 cells antioxidation |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030095 |
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