Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures

Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures

Summary: Here, we present a protocol for generating long-term microglia-containing air-liquid-interface cortical organoid (MG-ALI-CO) cultures. This approach minimizes necrotic core formation, a common limitation of extended organoid cultures, favoring microglia survival and homeostasis. We describe...

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Bibliographic Details
Main Authors: Marta Cañizares Luna, Mayte Mars, Channa E. Jakobs, Christiaan F.M. Huffels, Arthur Ermakov, Elly M. Hol, R. Jeroen Pasterkamp
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Immunology
Molecular Biology
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003211
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http://www.sciencedirect.com/science/article/pii/S2666166725003211

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