Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a

Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a

Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RS...

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Bibliographic Details
Main Authors: Ariya Khamwut, Juthamas Nimnual, Nantinee Chomta, Pattaraporn Nimsamer, Oraphan Mayuramart, Pornchai Kaewsapsak, Siripat Pasittungkul, Yong Poovorawan, Sunchai Payungporn
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Experimental Biology and Medicine
Subjects:
CRISPR-Cas12a
detection
isothermal amplification
RT-RPA
respiratory syncytial virus
Online Access:https://www.ebm-journal.org/articles/10.3389/ebm.2025.10387/full
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https://www.ebm-journal.org/articles/10.3389/ebm.2025.10387/full

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