Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections
BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) poses a significant challenge in clinical environments due to its resistance to standard antibiotics. Staphylococcal Protein A (SpA), a crucial virulence factor of MRSA, undermines host immune responses, making it an attractive target for...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1503944/full |
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| author | Pengjun Zhou Pengjun Zhou Xing Shi Jinquan Xia Yifei Wang Shaowei Dong |
| author_facet | Pengjun Zhou Pengjun Zhou Xing Shi Jinquan Xia Yifei Wang Shaowei Dong |
| author_sort | Pengjun Zhou |
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| description | BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) poses a significant challenge in clinical environments due to its resistance to standard antibiotics. Staphylococcal Protein A (SpA), a crucial virulence factor of MRSA, undermines host immune responses, making it an attractive target for vaccine development. This study aimed to identify potential epitopes within SpA that could elicit robust immune responses, ultimately contributing to the combat against multidrug-resistant (MDR) MRSA.MethodsThe SpA protein sequence was retrieved from the UniProt database, with various bioinformatics tools employed for epitope prediction. T-cell epitopes were identified using the Tepitool server, focusing on high-affinity interactions with prevalent human leukocyte antigens (HLAs). B-cell epitopes were predicted using the BepiPred tool. Predicted epitopes underwent docking studies with HLA molecules to evaluate binding properties. In-silico analyses confirmed the antigenicity, promiscuity, and non-glycosylated nature of the selected epitopes.ResultsSeveral T and B cell epitopes within SpA were identified, showcasing high binding affinities and extensive population coverage. A multi-epitope vaccine construct, linked by synthetic linkers and an adjuvant, was modelled, refined, and validated through various bioinformatics assessments. The vaccine candidate was subsequently docked with Toll-like receptor 4 (TLR-4) to evaluate its potential for immunogenicity.ConclusionThis study lays the groundwork for developing epitope-based vaccines targeting SpA in MRSA, identifying promising candidates for experimental validation and contributing to innovative immunotherapeutic strategies against MRSA infections. |
| format | Article |
| id | doaj-art-5ca4d58245024a198e150f53183abf7c |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-5ca4d58245024a198e150f53183abf7c2025-01-14T06:10:35ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.15039441503944Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infectionsPengjun Zhou0Pengjun Zhou1Xing Shi2Jinquan Xia3Yifei Wang4Shaowei Dong5Department of Pharmacology, Guangdong Pharmaceutical University, Guangzhou, ChinaGuangzhou Jinan Biomedicine Research and Development Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, ChinaDepartment of Haematology and Oncology, Shenzhen Children’s Hospital, Shenzhen, ChinaDepartment of Haematology and Oncology, Shenzhen Children’s Hospital, Shenzhen, ChinaGuangzhou Jinan Biomedicine Research and Development Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, ChinaDepartment of Haematology and Oncology, Shenzhen Children’s Hospital, Shenzhen, ChinaBackgroundMethicillin-resistant Staphylococcus aureus (MRSA) poses a significant challenge in clinical environments due to its resistance to standard antibiotics. Staphylococcal Protein A (SpA), a crucial virulence factor of MRSA, undermines host immune responses, making it an attractive target for vaccine development. This study aimed to identify potential epitopes within SpA that could elicit robust immune responses, ultimately contributing to the combat against multidrug-resistant (MDR) MRSA.MethodsThe SpA protein sequence was retrieved from the UniProt database, with various bioinformatics tools employed for epitope prediction. T-cell epitopes were identified using the Tepitool server, focusing on high-affinity interactions with prevalent human leukocyte antigens (HLAs). B-cell epitopes were predicted using the BepiPred tool. Predicted epitopes underwent docking studies with HLA molecules to evaluate binding properties. In-silico analyses confirmed the antigenicity, promiscuity, and non-glycosylated nature of the selected epitopes.ResultsSeveral T and B cell epitopes within SpA were identified, showcasing high binding affinities and extensive population coverage. A multi-epitope vaccine construct, linked by synthetic linkers and an adjuvant, was modelled, refined, and validated through various bioinformatics assessments. The vaccine candidate was subsequently docked with Toll-like receptor 4 (TLR-4) to evaluate its potential for immunogenicity.ConclusionThis study lays the groundwork for developing epitope-based vaccines targeting SpA in MRSA, identifying promising candidates for experimental validation and contributing to innovative immunotherapeutic strategies against MRSA infections.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1503944/fullhuman leukocyte antigenimmuno-informaticsmethicillin-resistant Staphylococcus aureusStaphylococcal Protein Atoll-like receptorvaccine development |
| spellingShingle | Pengjun Zhou Pengjun Zhou Xing Shi Jinquan Xia Yifei Wang Shaowei Dong Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections Frontiers in Cellular and Infection Microbiology human leukocyte antigen immuno-informatics methicillin-resistant Staphylococcus aureus Staphylococcal Protein A toll-like receptor vaccine development |
| title | Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections |
| title_full | Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections |
| title_fullStr | Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections |
| title_full_unstemmed | Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections |
| title_short | Innovative epitopes in Staphylococcal Protein-A an immuno-informatics approach to combat MDR-MRSA infections |
| title_sort | innovative epitopes in staphylococcal protein a an immuno informatics approach to combat mdr mrsa infections |
| topic | human leukocyte antigen immuno-informatics methicillin-resistant Staphylococcus aureus Staphylococcal Protein A toll-like receptor vaccine development |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1503944/full |
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