Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militaris
In the present study, the fruiting bodies of Cordyceps militaris were used as raw materials to extract the crude polysaccharides Y-1, Y-2, Y-3, Y-4, Y-5, Y-6 and Y-7, which fractional precipitation using the ethanol with different volume fractions of 30%, 40%, 50%, 60%, 70%, 80% and 90%. The graded...
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The editorial department of Science and Technology of Food Industry
2025-05-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070386 |
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| author | Yanan WANG Xinyi QIAN Yidan YONG Mengmeng WU Yihao LI Yuhang CHEN Zaizhong NI Lulu LI Anhui CHEN Peng ZHANG Ying GENG Ying SHAO |
| author_facet | Yanan WANG Xinyi QIAN Yidan YONG Mengmeng WU Yihao LI Yuhang CHEN Zaizhong NI Lulu LI Anhui CHEN Peng ZHANG Ying GENG Ying SHAO |
| author_sort | Yanan WANG |
| collection | DOAJ |
| description | In the present study, the fruiting bodies of Cordyceps militaris were used as raw materials to extract the crude polysaccharides Y-1, Y-2, Y-3, Y-4, Y-5, Y-6 and Y-7, which fractional precipitation using the ethanol with different volume fractions of 30%, 40%, 50%, 60%, 70%, 80% and 90%. The graded crude polysaccharide with the strongest antioxidant activity was selected to separate and purify the homogeneous polysaccharide with the strongest by DEAE-52 cellulose ion exchange chromatography and Sephadex G-150 gel chromatography. High performance gel chromatography, gas chromatography and infrared spectroscopy were used to identify the molecular weight, monosaccharide composition and functional groups of the homogeneous polysaccharide, and evaluate its antioxidant activity in vivo. The results showed that the graded polysaccharide Y-4 with the strongest antioxidant activity was isolated and purified by DEAE-52 cellulose ion exchange chromatography and Sephadex G-150 gel chromatography, and obtained a homogeneous polysaccharide STP-4 with the strongest antioxidant activity. STP-4 was an α-type configuration oligosaccharide composed of arabinose, mannose, and glucose, with a molecular weight of approximately 1434 Da. It had the functional groups such as O-H, C-H, COOH, C=O, and C-O-C. In addition, STP-4 could significantly reduce the malondialdehyde (MDA) content (P<0.01), increase the superoxide dismutase (SOD) activity (P<0.5), and the glutathione peroxidase (GSH-Px) activity (P<0.01) in the serum of mice with oxidative damage. This study provides a theoretical basis for the in-depth development and antioxidant activity research of Cordyceps militaris resources. |
| format | Article |
| id | doaj-art-5c826bae0cdb4847b4f8e047a8ed83cc |
| institution | DOAJ |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-5c826bae0cdb4847b4f8e047a8ed83cc2025-08-20T03:14:19ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-05-01469314010.13386/j.issn1002-0306.20240703862024070386-9Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militarisYanan WANG0Xinyi QIAN1Yidan YONG2Mengmeng WU3Yihao LI4Yuhang CHEN5Zaizhong NI6Lulu LI7Anhui CHEN8Peng ZHANG9Ying GENG10Ying SHAO11College of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaXuzhou Tianyi Food Technology Research Institute Co., Ltd., Xuzhou 221018, ChinaXuzhou Tianyi Food Technology Research Institute Co., Ltd., Xuzhou 221018, ChinaCollege of Food and Bioengineering, Xuzhou University of Technology, Xuzhou 221018, ChinaIn the present study, the fruiting bodies of Cordyceps militaris were used as raw materials to extract the crude polysaccharides Y-1, Y-2, Y-3, Y-4, Y-5, Y-6 and Y-7, which fractional precipitation using the ethanol with different volume fractions of 30%, 40%, 50%, 60%, 70%, 80% and 90%. The graded crude polysaccharide with the strongest antioxidant activity was selected to separate and purify the homogeneous polysaccharide with the strongest by DEAE-52 cellulose ion exchange chromatography and Sephadex G-150 gel chromatography. High performance gel chromatography, gas chromatography and infrared spectroscopy were used to identify the molecular weight, monosaccharide composition and functional groups of the homogeneous polysaccharide, and evaluate its antioxidant activity in vivo. The results showed that the graded polysaccharide Y-4 with the strongest antioxidant activity was isolated and purified by DEAE-52 cellulose ion exchange chromatography and Sephadex G-150 gel chromatography, and obtained a homogeneous polysaccharide STP-4 with the strongest antioxidant activity. STP-4 was an α-type configuration oligosaccharide composed of arabinose, mannose, and glucose, with a molecular weight of approximately 1434 Da. It had the functional groups such as O-H, C-H, COOH, C=O, and C-O-C. In addition, STP-4 could significantly reduce the malondialdehyde (MDA) content (P<0.01), increase the superoxide dismutase (SOD) activity (P<0.5), and the glutathione peroxidase (GSH-Px) activity (P<0.01) in the serum of mice with oxidative damage. This study provides a theoretical basis for the in-depth development and antioxidant activity research of Cordyceps militaris resources.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070386cordyceps militarisfractionated polysaccharidesantioxidantpurificationstructural characterization |
| spellingShingle | Yanan WANG Xinyi QIAN Yidan YONG Mengmeng WU Yihao LI Yuhang CHEN Zaizhong NI Lulu LI Anhui CHEN Peng ZHANG Ying GENG Ying SHAO Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militaris Shipin gongye ke-ji cordyceps militaris fractionated polysaccharides antioxidant purification structural characterization |
| title | Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militaris |
| title_full | Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militaris |
| title_fullStr | Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militaris |
| title_full_unstemmed | Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militaris |
| title_short | Isolation, Purification, and Structural Characterization of Antioxidant Polysaccharides Isolated from the Fruiting Bodies of Cordyceps militaris |
| title_sort | isolation purification and structural characterization of antioxidant polysaccharides isolated from the fruiting bodies of cordyceps militaris |
| topic | cordyceps militaris fractionated polysaccharides antioxidant purification structural characterization |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024070386 |
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