Precision RNAi using synthetic shRNAmir target sites
Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/84792 |
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| author | Thomas Hoffmann Alexandra Hörmann Maja Corcokovic Jakub Zmajkovic Matthias Hinterndorfer Jasko Salkanovic Fiona Spreitzer Anna Köferle Katrin Gitschtaler Alexandra Popa Sarah Oberndorfer Florian Andersch Markus Schaefer Michaela Fellner Nicole Budano Jan G Ruppert Paolo Chetta Melanie Wurm Johannes Zuber Ralph A Neumüller |
| author_facet | Thomas Hoffmann Alexandra Hörmann Maja Corcokovic Jakub Zmajkovic Matthias Hinterndorfer Jasko Salkanovic Fiona Spreitzer Anna Köferle Katrin Gitschtaler Alexandra Popa Sarah Oberndorfer Florian Andersch Markus Schaefer Michaela Fellner Nicole Budano Jan G Ruppert Paolo Chetta Melanie Wurm Johannes Zuber Ralph A Neumüller |
| author_sort | Thomas Hoffmann |
| collection | DOAJ |
| description | Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient and inducible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects. |
| format | Article |
| id | doaj-art-5be3978b927a49d695b800fdcd6cb2bb |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2023-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-5be3978b927a49d695b800fdcd6cb2bb2025-08-20T03:48:14ZengeLife Sciences Publications LtdeLife2050-084X2023-08-011210.7554/eLife.84792Precision RNAi using synthetic shRNAmir target sitesThomas Hoffmann0https://orcid.org/0000-0002-1589-0013Alexandra Hörmann1Maja Corcokovic2Jakub Zmajkovic3https://orcid.org/0000-0001-7061-6538Matthias Hinterndorfer4https://orcid.org/0000-0003-2435-4690Jasko Salkanovic5Fiona Spreitzer6Anna Köferle7https://orcid.org/0000-0003-1601-7774Katrin Gitschtaler8Alexandra Popa9Sarah Oberndorfer10Florian Andersch11Markus Schaefer12Michaela Fellner13Nicole Budano14Jan G Ruppert15Paolo Chetta16Melanie Wurm17Johannes Zuber18https://orcid.org/0000-0001-8810-6835Ralph A Neumüller19https://orcid.org/0000-0002-1514-6278Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna, Austria; Vienna BioCenter PhD Program, Doctoral School of the University at Vienna and Medical University of Vienna, Vienna BioCenter (VBC), Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaResearch Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna, AustriaResearch Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaResearch Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna, AustriaResearch Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna, AustriaResearch Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaResearch Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, Vienna, Austria; Medical University of Vienna, Vienna BioCenter, Vienna, AustriaBoehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse, Vienna, AustriaLoss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient and inducible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.https://elifesciences.org/articles/84792cancerRNAiKRASEGFRtarget validationloss-of-function |
| spellingShingle | Thomas Hoffmann Alexandra Hörmann Maja Corcokovic Jakub Zmajkovic Matthias Hinterndorfer Jasko Salkanovic Fiona Spreitzer Anna Köferle Katrin Gitschtaler Alexandra Popa Sarah Oberndorfer Florian Andersch Markus Schaefer Michaela Fellner Nicole Budano Jan G Ruppert Paolo Chetta Melanie Wurm Johannes Zuber Ralph A Neumüller Precision RNAi using synthetic shRNAmir target sites eLife cancer RNAi KRAS EGFR target validation loss-of-function |
| title | Precision RNAi using synthetic shRNAmir target sites |
| title_full | Precision RNAi using synthetic shRNAmir target sites |
| title_fullStr | Precision RNAi using synthetic shRNAmir target sites |
| title_full_unstemmed | Precision RNAi using synthetic shRNAmir target sites |
| title_short | Precision RNAi using synthetic shRNAmir target sites |
| title_sort | precision rnai using synthetic shrnamir target sites |
| topic | cancer RNAi KRAS EGFR target validation loss-of-function |
| url | https://elifesciences.org/articles/84792 |
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