Precision RNAi using synthetic shRNAmir target sites

Precision RNAi using synthetic shRNAmir target sites

Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient...

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Bibliographic Details
Main Authors: Thomas Hoffmann, Alexandra Hörmann, Maja Corcokovic, Jakub Zmajkovic, Matthias Hinterndorfer, Jasko Salkanovic, Fiona Spreitzer, Anna Köferle, Katrin Gitschtaler, Alexandra Popa, Sarah Oberndorfer, Florian Andersch, Markus Schaefer, Michaela Fellner, Nicole Budano, Jan G Ruppert, Paolo Chetta, Melanie Wurm, Johannes Zuber, Ralph A Neumüller
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2023-08-01
Series:eLife
Subjects:
cancer
RNAi
KRAS
EGFR
target validation
loss-of-function
Online Access:https://elifesciences.org/articles/84792
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https://elifesciences.org/articles/84792

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