A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye

We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated fluorescein dye that is taken up by apoptotic cells at the stage of phosphatid...

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Bibliographic Details
Main Authors: Mervin Meyer, Magbubah Essack, Stonard Kanyanda, Jasper G. Rees
Format: Article
Language:English
Published: Taylor & Francis Group 2008-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112908
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