Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>)
To assess the impact of tannic acid (TA) on the hepatic health of the Chinese soft-shelled turtle, the individuals were fed diets containing 0 (CG), 0.5, 1, 2, and 4 g/kg TA diets for 60 days (0 hps). Afterwards, the turtles were challenged with 15 °C cold stress for 24 h (24 hps) and then recovered...
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2025-02-01
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| author | Liqin Ji Yisen Shangguan Qing Shi Zhen Dong Chen Chen Junxian Zhu Xiaoyou Hong Xiaoli Liu Chengqing Wei Xinping Zhu Wei Li |
| author_facet | Liqin Ji Yisen Shangguan Qing Shi Zhen Dong Chen Chen Junxian Zhu Xiaoyou Hong Xiaoli Liu Chengqing Wei Xinping Zhu Wei Li |
| author_sort | Liqin Ji |
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| description | To assess the impact of tannic acid (TA) on the hepatic health of the Chinese soft-shelled turtle, the individuals were fed diets containing 0 (CG), 0.5, 1, 2, and 4 g/kg TA diets for 60 days (0 hps). Afterwards, the turtles were challenged with 15 °C cold stress for 24 h (24 hps) and then recovered to 28 °C for 24 h (48 hps). The results indicated that 4 g/kg TA has a significant toxic effect on the turtles after 60-day administration. The hepatic T-SOD, CAT, GSH-Px, and T-AOC activities in the TA2 were increased at 0 hps and 24 hps (<i>p</i> < 0.05) compared with CG. Similarly, <i>Sod1</i>, <i>Sod2</i>, <i>Cat</i>, <i>Gsh-px3</i>, and <i>Gsh-px4</i> mRNA levels in the TA2 were higher than in the other four groups at 0 hps and 24 hps (<i>p</i> < 0.05). The histology data showed that 4 g/kg TA induced injuries in liver at 0 hps and after temperature fluctuation, whereas the 2 g/kg TA effectively attenuated the hepatic injuries. A total of 202 differentially expressed metabolites (DEMs) in the CG vs. TA2 and 115 DEMs in the LTCG vs. LTTA2 were separately detected by the metabolome. The cephalosporin C, protoporphyrin, bis(2-ethylhexyl) phthalate, 2-acetamidoethylphosphonat, verbasosid, and norcocain, were obvious DEMs in the CG vs. TA2. Furthermore, valienone 7-phosphate, 5-HETE, pregnanetriolone, 4-keto-anhydrotetracyclin, dephospho-CoA, and lysoPC(18:4(6Z,9Z,12Z,15Z)/0:0) were top DEMs in the LTCG vs. LTTA2. The “adipocytokine signaling pathway” and “AMPK signaling pathway” were the key pathways enriched in the CG vs. TA2, while “fatty acid biosynthesis”, “steroid biosynthesis”, and “linoleic acid metabolism” were most abundant in the LTCG vs. LTTA2. Generally, this research indicated that 2 g/kg TA could protect hepatic health from temperature fluctuations by improving antioxidant capacity, reducing histological injuries, and regulating lipid-related signaling pathways. |
| format | Article |
| id | doaj-art-5aa3606651ed4151be1d0dedeabf1c8e |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-5aa3606651ed4151be1d0dedeabf1c8e2025-08-20T03:11:07ZengMDPI AGAnimals2076-26152025-02-0115454410.3390/ani15040544Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>)Liqin Ji0Yisen Shangguan1Qing Shi2Zhen Dong3Chen Chen4Junxian Zhu5Xiaoyou Hong6Xiaoli Liu7Chengqing Wei8Xinping Zhu9Wei Li10Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Marine Environmental Survey Technology and Application, South China Sea Marine Survey Center, Ministry of Natural Resources, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaKey Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510275, ChinaTo assess the impact of tannic acid (TA) on the hepatic health of the Chinese soft-shelled turtle, the individuals were fed diets containing 0 (CG), 0.5, 1, 2, and 4 g/kg TA diets for 60 days (0 hps). Afterwards, the turtles were challenged with 15 °C cold stress for 24 h (24 hps) and then recovered to 28 °C for 24 h (48 hps). The results indicated that 4 g/kg TA has a significant toxic effect on the turtles after 60-day administration. The hepatic T-SOD, CAT, GSH-Px, and T-AOC activities in the TA2 were increased at 0 hps and 24 hps (<i>p</i> < 0.05) compared with CG. Similarly, <i>Sod1</i>, <i>Sod2</i>, <i>Cat</i>, <i>Gsh-px3</i>, and <i>Gsh-px4</i> mRNA levels in the TA2 were higher than in the other four groups at 0 hps and 24 hps (<i>p</i> < 0.05). The histology data showed that 4 g/kg TA induced injuries in liver at 0 hps and after temperature fluctuation, whereas the 2 g/kg TA effectively attenuated the hepatic injuries. A total of 202 differentially expressed metabolites (DEMs) in the CG vs. TA2 and 115 DEMs in the LTCG vs. LTTA2 were separately detected by the metabolome. The cephalosporin C, protoporphyrin, bis(2-ethylhexyl) phthalate, 2-acetamidoethylphosphonat, verbasosid, and norcocain, were obvious DEMs in the CG vs. TA2. Furthermore, valienone 7-phosphate, 5-HETE, pregnanetriolone, 4-keto-anhydrotetracyclin, dephospho-CoA, and lysoPC(18:4(6Z,9Z,12Z,15Z)/0:0) were top DEMs in the LTCG vs. LTTA2. The “adipocytokine signaling pathway” and “AMPK signaling pathway” were the key pathways enriched in the CG vs. TA2, while “fatty acid biosynthesis”, “steroid biosynthesis”, and “linoleic acid metabolism” were most abundant in the LTCG vs. LTTA2. Generally, this research indicated that 2 g/kg TA could protect hepatic health from temperature fluctuations by improving antioxidant capacity, reducing histological injuries, and regulating lipid-related signaling pathways.https://www.mdpi.com/2076-2615/15/4/544Chinese soft-shelled turtlelivertannin acidhistologymetabolitessignaling pathways |
| spellingShingle | Liqin Ji Yisen Shangguan Qing Shi Zhen Dong Chen Chen Junxian Zhu Xiaoyou Hong Xiaoli Liu Chengqing Wei Xinping Zhu Wei Li Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) Animals Chinese soft-shelled turtle liver tannin acid histology metabolites signaling pathways |
| title | Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) |
| title_full | Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) |
| title_fullStr | Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) |
| title_full_unstemmed | Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) |
| title_short | Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) |
| title_sort | dietary tannic acid improves hepatic health and capacity to deal with temperature fluctuation in the chinese soft shelled turtle i pelodiscus sinensis i |
| topic | Chinese soft-shelled turtle liver tannin acid histology metabolites signaling pathways |
| url | https://www.mdpi.com/2076-2615/15/4/544 |
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