Unlocking efficiency in column chromatography with packed bed supporting inserts
To purify increasing amounts of biotherapeutics more efficiently, the use of high flow rates or greater resin bed heights during downstream chromatography steps is a tantalizing option. A limitation of utilizing high flow rates is the differential pressure generated by packed chromatography resin be...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1613174/full |
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| author | Romone M. Fancy David H. Abraham Matthew R. Taylor Cole McMullin Nicholas S. Brann Daniel M. Bailey David I. Brown Heather Bethea Horne Leslie S. Wolfe |
| author_facet | Romone M. Fancy David H. Abraham Matthew R. Taylor Cole McMullin Nicholas S. Brann Daniel M. Bailey David I. Brown Heather Bethea Horne Leslie S. Wolfe |
| author_sort | Romone M. Fancy |
| collection | DOAJ |
| description | To purify increasing amounts of biotherapeutics more efficiently, the use of high flow rates or greater resin bed heights during downstream chromatography steps is a tantalizing option. A limitation of utilizing high flow rates is the differential pressure generated by packed chromatography resin beds. As a resin bed height increases, the resin is susceptible to compression. By increasing the permeability of a packed resin bed through control of the hydraulic radius, column pressure-flow dynamics can be improved. Chromatography column performance using a commercially available Protein A resin was assessed with and without OMEGA, a column insert designed to modulate the hydraulic radius of the column by providing vertical supports through the packed resin bed. OMEGA was shown to reduce the effective hydraulic radius of packed resin beds, increase the permeability of packed columns by 44%–73%, and yield a 42%–50% decrease in pressure differential across the resin bed at a comparable linear velocity. The structural support provided by OMEGA enables higher operational flow rates and increased resin bed height without impact to either dynamic binding capacity or purified product quality. With the OMEGA column insert, scale-up hurdles are mitigated, and faster downstream processing times are unlocked across column geometries. |
| format | Article |
| id | doaj-art-5a9f89ac5fe64950af07d23e8fdb68b7 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-5a9f89ac5fe64950af07d23e8fdb68b72025-08-20T03:24:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-06-011310.3389/fbioe.2025.16131741613174Unlocking efficiency in column chromatography with packed bed supporting insertsRomone M. Fancy0David H. Abraham1Matthew R. Taylor2Cole McMullin3Nicholas S. Brann4Daniel M. Bailey5David I. Brown6Heather Bethea Horne7Leslie S. Wolfe8KBI Biopharma, Durham, NC, United StatesKBI Biopharma, Durham, NC, United StatesKBI Biopharma, Durham, NC, United StatesKBI Biopharma, Durham, NC, United StatesKBI Biopharma, Durham, NC, United StatesJSR Life Sciences, Sunnyvale, CA, United StatesKBI Biopharma, Durham, NC, United StatesKBI Biopharma, Durham, NC, United StatesKBI Biopharma, Durham, NC, United StatesTo purify increasing amounts of biotherapeutics more efficiently, the use of high flow rates or greater resin bed heights during downstream chromatography steps is a tantalizing option. A limitation of utilizing high flow rates is the differential pressure generated by packed chromatography resin beds. As a resin bed height increases, the resin is susceptible to compression. By increasing the permeability of a packed resin bed through control of the hydraulic radius, column pressure-flow dynamics can be improved. Chromatography column performance using a commercially available Protein A resin was assessed with and without OMEGA, a column insert designed to modulate the hydraulic radius of the column by providing vertical supports through the packed resin bed. OMEGA was shown to reduce the effective hydraulic radius of packed resin beds, increase the permeability of packed columns by 44%–73%, and yield a 42%–50% decrease in pressure differential across the resin bed at a comparable linear velocity. The structural support provided by OMEGA enables higher operational flow rates and increased resin bed height without impact to either dynamic binding capacity or purified product quality. With the OMEGA column insert, scale-up hurdles are mitigated, and faster downstream processing times are unlocked across column geometries.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1613174/fullresin bedchromatographybiomanufacturingantibodypurificationpermeability |
| spellingShingle | Romone M. Fancy David H. Abraham Matthew R. Taylor Cole McMullin Nicholas S. Brann Daniel M. Bailey David I. Brown Heather Bethea Horne Leslie S. Wolfe Unlocking efficiency in column chromatography with packed bed supporting inserts Frontiers in Bioengineering and Biotechnology resin bed chromatography biomanufacturing antibody purification permeability |
| title | Unlocking efficiency in column chromatography with packed bed supporting inserts |
| title_full | Unlocking efficiency in column chromatography with packed bed supporting inserts |
| title_fullStr | Unlocking efficiency in column chromatography with packed bed supporting inserts |
| title_full_unstemmed | Unlocking efficiency in column chromatography with packed bed supporting inserts |
| title_short | Unlocking efficiency in column chromatography with packed bed supporting inserts |
| title_sort | unlocking efficiency in column chromatography with packed bed supporting inserts |
| topic | resin bed chromatography biomanufacturing antibody purification permeability |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1613174/full |
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