The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis loosening
Abstract Background Aseptic prosthesis loosening caused by wear particles is a major complication in patients with osteoarthritis following total joint replacement. Despite advancements in treatment, the underlying mechanisms remain poorly understood, and effective therapies are still lacking. Metho...
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| Language: | English |
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BMC
2025-03-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02452-3 |
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| author | Ping Chen Hao-Hui Guo Long Wu Shuai Zhang Qun-Hua Jin Ke-Ning Sun |
| author_facet | Ping Chen Hao-Hui Guo Long Wu Shuai Zhang Qun-Hua Jin Ke-Ning Sun |
| author_sort | Ping Chen |
| collection | DOAJ |
| description | Abstract Background Aseptic prosthesis loosening caused by wear particles is a major complication in patients with osteoarthritis following total joint replacement. Despite advancements in treatment, the underlying mechanisms remain poorly understood, and effective therapies are still lacking. Methods In this study, we investigated the effects of titanium particles on osteoclast and osteoblast differentiation through both in vitro and in vivo experiments. Results Our findings revealed that titanium particles not only promote the differentiation of RAW264.7 cells into osteoclasts and enhance the secretion of inflammatory factors but also inhibit the differentiation of BMSCs into osteoblasts and reduce the expression of Wnt signaling pathway-related factors. Furthermore, using a mouse model of knee prosthesis loosening and AAV-mediated gene therapy, we demonstrated that the combination of TNF-α interference and Wnt3a overexpression was more effective than single-gene therapy in reducing inflammatory cell infiltration, promoting bone formation, and mitigating bone destruction. Conclusions These results highlight the dual role of titanium particles in periprosthetic osteolysis and underscore the potential of a gene therapy strategy targeting both inflammatory factors and the Wnt signaling pathway to improve knee prosthesis loosening. This study provides a scientific foundation for developing novel therapeutic approaches. |
| format | Article |
| id | doaj-art-5a94b194057b4c28aa124ccc2c46f8f6 |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-5a94b194057b4c28aa124ccc2c46f8f62025-08-20T03:41:12ZengBMCEuropean Journal of Medical Research2047-783X2025-03-013011910.1186/s40001-025-02452-3The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis looseningPing Chen0Hao-Hui Guo1Long Wu2Shuai Zhang3Qun-Hua Jin4Ke-Ning Sun5Medical Experiment Center, General Hospital of Ningxia Medical UniversityOrthopedics Ward 3, The General Hospital of Ningxia Medical UniversityOrthopedics Ward 3, The General Hospital of Ningxia Medical UniversityOrthopedics Ward 3, The General Hospital of Ningxia Medical UniversityOrthopedics Ward 3, The General Hospital of Ningxia Medical UniversityOrthopedics Ward 3, The General Hospital of Ningxia Medical UniversityAbstract Background Aseptic prosthesis loosening caused by wear particles is a major complication in patients with osteoarthritis following total joint replacement. Despite advancements in treatment, the underlying mechanisms remain poorly understood, and effective therapies are still lacking. Methods In this study, we investigated the effects of titanium particles on osteoclast and osteoblast differentiation through both in vitro and in vivo experiments. Results Our findings revealed that titanium particles not only promote the differentiation of RAW264.7 cells into osteoclasts and enhance the secretion of inflammatory factors but also inhibit the differentiation of BMSCs into osteoblasts and reduce the expression of Wnt signaling pathway-related factors. Furthermore, using a mouse model of knee prosthesis loosening and AAV-mediated gene therapy, we demonstrated that the combination of TNF-α interference and Wnt3a overexpression was more effective than single-gene therapy in reducing inflammatory cell infiltration, promoting bone formation, and mitigating bone destruction. Conclusions These results highlight the dual role of titanium particles in periprosthetic osteolysis and underscore the potential of a gene therapy strategy targeting both inflammatory factors and the Wnt signaling pathway to improve knee prosthesis loosening. This study provides a scientific foundation for developing novel therapeutic approaches.https://doi.org/10.1186/s40001-025-02452-3Aseptic prosthesis looseningTitanium particlesOsteolysisGene therapyTNF-αWnt3a |
| spellingShingle | Ping Chen Hao-Hui Guo Long Wu Shuai Zhang Qun-Hua Jin Ke-Ning Sun The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis loosening European Journal of Medical Research Aseptic prosthesis loosening Titanium particles Osteolysis Gene therapy TNF-α Wnt3a |
| title | The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis loosening |
| title_full | The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis loosening |
| title_fullStr | The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis loosening |
| title_full_unstemmed | The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis loosening |
| title_short | The dual role of titanium particles in osteolysis: implications for gene therapy in prosthesis loosening |
| title_sort | dual role of titanium particles in osteolysis implications for gene therapy in prosthesis loosening |
| topic | Aseptic prosthesis loosening Titanium particles Osteolysis Gene therapy TNF-α Wnt3a |
| url | https://doi.org/10.1186/s40001-025-02452-3 |
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