Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse
Fourier transform infrared spectroscopic imaging (FTIRI) was used to study bone healing with spatial analysis of various callus tissues in wild type mice. Femoral fractures were produced in 28 male C57BL mice by osteotomy. Animals were sacrificed at 1, 2, 4, and 8 weeks to obtain callus tissue at we...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2015/659473 |
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| author | Hans Gollwitzer Xu Yang Lyudmila Spevak Lyudmila Lukashova Allina Nocon Kara Fields Nancy Pleshko Hayden William Courtland Mathias P. Bostrom Adele L. Boskey |
| author_facet | Hans Gollwitzer Xu Yang Lyudmila Spevak Lyudmila Lukashova Allina Nocon Kara Fields Nancy Pleshko Hayden William Courtland Mathias P. Bostrom Adele L. Boskey |
| author_sort | Hans Gollwitzer |
| collection | DOAJ |
| description | Fourier transform infrared spectroscopic imaging (FTIRI) was used to study bone healing with spatial analysis of various callus tissues in wild type mice. Femoral fractures were produced in 28 male C57BL mice by osteotomy. Animals were sacrificed at 1, 2, 4, and 8 weeks to obtain callus tissue at well-defined healing stages. Following microcomputerized tomography, bone samples were cut in consecutive sections for FTIRI and histology, allowing for spatial correlation of both imaging methods in different callus areas (early calcified cartilage, woven bone, areas of intramembranous and endochondral bone formation). Based on FTIRI, mineral/matrix ratio increased significantly during the first 4 weeks of fracture healing in all callus areas and correlated with bone mineral density measured by micro-CT. Carbonate/phosphate ratio was elevated in newly formed calcified tissue and at week 2 attained values comparable to cortical bone. Collagen maturity and mineral crystallinity increased during weeks 1–8 in most tissues while acid phosphate substitution decreased. Temporal and callus area dependent changes were detected throughout the healing period. These data assert the usefulness of FTIRI for evaluation of fracture healing in the mouse and its potential to evaluate pathologic fracture healing and the effects of therapeutic interventions. |
| format | Article |
| id | doaj-art-5a8032e4fede4acab991359fbe3f8e64 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-5a8032e4fede4acab991359fbe3f8e642025-02-03T01:10:59ZengWileyJournal of Spectroscopy2314-49202314-49392015-01-01201510.1155/2015/659473659473Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal MouseHans Gollwitzer0Xu Yang1Lyudmila Spevak2Lyudmila Lukashova3Allina Nocon4Kara Fields5Nancy Pleshko6Hayden William Courtland7Mathias P. Bostrom8Adele L. Boskey9Hospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USATemple University College of Engineering, 1947 N. 12th Street, Philadelphia, PA 19122, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAHospital for Special Surgery, 535 E. 70th Street, New York, NY 10021, USAFourier transform infrared spectroscopic imaging (FTIRI) was used to study bone healing with spatial analysis of various callus tissues in wild type mice. Femoral fractures were produced in 28 male C57BL mice by osteotomy. Animals were sacrificed at 1, 2, 4, and 8 weeks to obtain callus tissue at well-defined healing stages. Following microcomputerized tomography, bone samples were cut in consecutive sections for FTIRI and histology, allowing for spatial correlation of both imaging methods in different callus areas (early calcified cartilage, woven bone, areas of intramembranous and endochondral bone formation). Based on FTIRI, mineral/matrix ratio increased significantly during the first 4 weeks of fracture healing in all callus areas and correlated with bone mineral density measured by micro-CT. Carbonate/phosphate ratio was elevated in newly formed calcified tissue and at week 2 attained values comparable to cortical bone. Collagen maturity and mineral crystallinity increased during weeks 1–8 in most tissues while acid phosphate substitution decreased. Temporal and callus area dependent changes were detected throughout the healing period. These data assert the usefulness of FTIRI for evaluation of fracture healing in the mouse and its potential to evaluate pathologic fracture healing and the effects of therapeutic interventions.http://dx.doi.org/10.1155/2015/659473 |
| spellingShingle | Hans Gollwitzer Xu Yang Lyudmila Spevak Lyudmila Lukashova Allina Nocon Kara Fields Nancy Pleshko Hayden William Courtland Mathias P. Bostrom Adele L. Boskey Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse Journal of Spectroscopy |
| title | Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse |
| title_full | Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse |
| title_fullStr | Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse |
| title_full_unstemmed | Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse |
| title_short | Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse |
| title_sort | fourier transform infrared spectroscopic imaging of fracture healing in the normal mouse |
| url | http://dx.doi.org/10.1155/2015/659473 |
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