Polymorphic Amplified Typing Sequences and Pulsed-Field Gel Electrophoresis Yield Comparable Results in the Strain Typing of a Diverse Set of Bovine Escherichia coli O157:H7 Isolates

Polymorphic amplified typing sequences (PATS), a PCR-based Escherichia coli O157:H7 (O157) strain typing system, targets insertions-deletions and single nucleotide polymorphisms at XbaI and AvrII restriction enzyme sites, respectively, and the virulence genes (stx1, stx2, eae, hlyA) in the O157 geno...

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Bibliographic Details
Main Authors: Indira T. Kudva, Margaret A. Davis, Robert W. Griffin, Jeonifer Garren, Megan Murray, Manohar John, Carolyn J. Hovde, Stephen B. Calderwood
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2012/140105
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