Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus Mice
Frost mulberry leaves possess significant medicinal and nutritional values and feature extensive resource availability and convenient acquisition. The study investigated the physicochemical structure and functional properties of frost mulberry leaf superfine powder (FMLSP) and the effects of FMLSP o...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/15/7/3766 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850212845076086784 |
|---|---|
| author | Jingya Wu Qiu Wu Guojian Zhao Jing Liang Lei Sun Ming Jia Rui Sun Mingguan Yang |
| author_facet | Jingya Wu Qiu Wu Guojian Zhao Jing Liang Lei Sun Ming Jia Rui Sun Mingguan Yang |
| author_sort | Jingya Wu |
| collection | DOAJ |
| description | Frost mulberry leaves possess significant medicinal and nutritional values and feature extensive resource availability and convenient acquisition. The study investigated the physicochemical structure and functional properties of frost mulberry leaf superfine powder (FMLSP) and the effects of FMLSP on the hypoglycemic activity and gut microbiota of type 2 diabetes mellitus (T2DM) mice. The results indicated that the total flavonoid content of FMLSP reached 91.30 mg/g, with significant inhibitory effects on both α-glucosidase and α-amylase activities. Animal experimental data showed that FMLSP could significantly reduce insulin content, improve insulin resistance, and protect liver and pancreatic tissues in T2DM mice. Meanwhile, FMLSP showed significant effects on lipid metabolism, especially the low-density lipoprotein cholesterol (LDL-C) content in T2DM mice was significantly reduced by 76.22%. In addition, FMLSP has excellent antioxidant effects, which greatly alleviated the oxidative stress phenomenon in T2DM mice, especially the malondialdehyde (MDA) content was significantly reduced by 72.17%. FMLSP also restored the diversity and structure of the gut microbiota, significantly increasing the abundance of beneficial bacteria such as <i>Akkermansia</i>, <i>Lachnospiraceae_NK4A136_group</i>, <i>Alloprevotella</i>, and <i>Lactobacillus</i> in T2DM mice and significantly decreasing the abundance of abundance of harmful bacteria such as <i>Rikenellaceae_RC9_gut_group</i>, <i>Enterorhabdus</i>. These results indicate that FMLSP may serve as a potential dietary intervention for the prevention and treatment of T2DM. |
| format | Article |
| id | doaj-art-585950ea07b249c58700e59654c00058 |
| institution | OA Journals |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-585950ea07b249c58700e59654c000582025-08-20T02:09:14ZengMDPI AGApplied Sciences2076-34172025-03-01157376610.3390/app15073766Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus MiceJingya Wu0Qiu Wu1Guojian Zhao2Jing Liang3Lei Sun4Ming Jia5Rui Sun6Mingguan Yang7School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaCollege of Life Sciences, Shandong Normal University, Jinan 250300, ChinaSchool of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaEconomic Forest Institute, Shandong Academy of Forestry Sciences, Jinan 250014, ChinaEconomic Forest Institute, Shandong Academy of Forestry Sciences, Jinan 250014, ChinaEconomic Forest Institute, Shandong Academy of Forestry Sciences, Jinan 250014, ChinaSchool of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaSchool of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaFrost mulberry leaves possess significant medicinal and nutritional values and feature extensive resource availability and convenient acquisition. The study investigated the physicochemical structure and functional properties of frost mulberry leaf superfine powder (FMLSP) and the effects of FMLSP on the hypoglycemic activity and gut microbiota of type 2 diabetes mellitus (T2DM) mice. The results indicated that the total flavonoid content of FMLSP reached 91.30 mg/g, with significant inhibitory effects on both α-glucosidase and α-amylase activities. Animal experimental data showed that FMLSP could significantly reduce insulin content, improve insulin resistance, and protect liver and pancreatic tissues in T2DM mice. Meanwhile, FMLSP showed significant effects on lipid metabolism, especially the low-density lipoprotein cholesterol (LDL-C) content in T2DM mice was significantly reduced by 76.22%. In addition, FMLSP has excellent antioxidant effects, which greatly alleviated the oxidative stress phenomenon in T2DM mice, especially the malondialdehyde (MDA) content was significantly reduced by 72.17%. FMLSP also restored the diversity and structure of the gut microbiota, significantly increasing the abundance of beneficial bacteria such as <i>Akkermansia</i>, <i>Lachnospiraceae_NK4A136_group</i>, <i>Alloprevotella</i>, and <i>Lactobacillus</i> in T2DM mice and significantly decreasing the abundance of abundance of harmful bacteria such as <i>Rikenellaceae_RC9_gut_group</i>, <i>Enterorhabdus</i>. These results indicate that FMLSP may serve as a potential dietary intervention for the prevention and treatment of T2DM.https://www.mdpi.com/2076-3417/15/7/3766frosted mulberry leafsuperfine grindinghypoglycemictype 2 diabetes micegut microbiota |
| spellingShingle | Jingya Wu Qiu Wu Guojian Zhao Jing Liang Lei Sun Ming Jia Rui Sun Mingguan Yang Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus Mice Applied Sciences frosted mulberry leaf superfine grinding hypoglycemic type 2 diabetes mice gut microbiota |
| title | Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus Mice |
| title_full | Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus Mice |
| title_fullStr | Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus Mice |
| title_full_unstemmed | Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus Mice |
| title_short | Effects of Frost Mulberry Leaf Superfine Powder on the Hypoglycemic and Gut Microbiota of High-Fat Diet/Streptozotocin-Induced Type 2 Diabetes Mellitus Mice |
| title_sort | effects of frost mulberry leaf superfine powder on the hypoglycemic and gut microbiota of high fat diet streptozotocin induced type 2 diabetes mellitus mice |
| topic | frosted mulberry leaf superfine grinding hypoglycemic type 2 diabetes mice gut microbiota |
| url | https://www.mdpi.com/2076-3417/15/7/3766 |
| work_keys_str_mv | AT jingyawu effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice AT qiuwu effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice AT guojianzhao effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice AT jingliang effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice AT leisun effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice AT mingjia effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice AT ruisun effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice AT mingguanyang effectsoffrostmulberryleafsuperfinepowderonthehypoglycemicandgutmicrobiotaofhighfatdietstreptozotocininducedtype2diabetesmellitusmice |