Optimized zymogram protocol from 3D spheroid cultures to study MMP-2 and -9 activities in tumor cells

Optimized zymogram protocol from 3D spheroid cultures to study MMP-2 and -9 activities in tumor cells

Abstract Three-dimensional spheroids are more representative of tumors than cell-cultured monolayers. As in tumors, gradients of oxygen, nutrients and wastes are found in spheroid cultures but not in classical cultured monolayers. On the other hand, cell-based assays on the latter are hardly applica...

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Bibliographic Details
Main Authors: Sandra Majo, Chloe Redoute-Timonnier, Aurelie Lacour, Laurine Challeat, Eva Epinette, Jeremie Teillon, Christophe F. Grosset, Patrick Auguste
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Biotechnology
Subjects:
Renal cancer cells
3D tumor model
Spheroid
Collagen I
MMP-2
MMP-9
Online Access:https://doi.org/10.1186/s12896-025-00961-x
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https://doi.org/10.1186/s12896-025-00961-x

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