Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression
PurposeTo study whether fecal microbiota transplantation (FMT) can alleviate lipopolysaccharide (LPS)-induced osteoporosis (OP) by regulating the composition and abundance of gut microbiota and the expression level of long non-coding RNA (lncRNA) TUG1.MethodsTwenty C57BL/6 mice were selected. Two mi...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1535666/full |
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| author | Pengcheng Ma Ruoyi Wang Huizhi Chen Jiachun Zheng Weijie Yang Bo Meng Yifan Liu Yao Lu Jing Zhao Hongwei Gao Hongwei Gao |
| author_facet | Pengcheng Ma Ruoyi Wang Huizhi Chen Jiachun Zheng Weijie Yang Bo Meng Yifan Liu Yao Lu Jing Zhao Hongwei Gao Hongwei Gao |
| author_sort | Pengcheng Ma |
| collection | DOAJ |
| description | PurposeTo study whether fecal microbiota transplantation (FMT) can alleviate lipopolysaccharide (LPS)-induced osteoporosis (OP) by regulating the composition and abundance of gut microbiota and the expression level of long non-coding RNA (lncRNA) TUG1.MethodsTwenty C57BL/6 mice were selected. Two mice were randomly designated as fecal donors, while the remaining mice were randomly divided into control group, LPS group, and LPS + FMT group. Each group consisted of 6 mice. The mice in the LPS and LPS + FMT groups were intraperitoneally injected with LPS to establish the OP model, and the mice in the LPS + FMT group were treated with donor feces by gavage. Micro-CT was used to scan the femur specimens of mice, and the bone structural parameters of the control and LPS groups were compared to verify the effectiveness of the OP model. HE staining was used to compare the microstructure of femurs in the 3 groups. 16S rRNA gene sequencing was used to analyze the composition and abundance of gut microbiota in mice. Immunofluorescence staining was used to compare the expression levels of Runt-related transcription factor 2 (RUNX2) in the femur of the 3 groups. Real-time quantitative reverse transcription PCR (qRT-PCR) was used to compare the expression levels of lncRNA TUG1 in the intestines and serum of mice in the 3 groups.ResultsMicro-CT showed that compared with the control group, the mice in the LPS group had more bone loss. The bone mineral density, trabecular number, and trabecular thickness of the control group was higher, and the trabecular separation was smaller. The models were validated effectively. HE staining showed that compared with the control group, the bone trabeculae in the LPS group were thinner and sparse, while that in the LPS + FMT group were dense and clear. The 16s rRNA sequencing showed that the abundance of Bacteroides and Lactobacillus in LPS+FMT group was significantly higher than that in LPS group. Immunofluorescence staining showed that the RUNX2 level in the control group and LPS + FMT group was similar, and both were higher than that in the LPS group. The qRT-PCR results showed that the TUG1 mRNA level in the control group and LPS + FMT group was similar and significantly higher than that in the LPS group.ConclusionFMT can enhance osteoblast levels and improve bone structure by modulating the abundance of gut microbiota in OP mice (such as increasing Bacteroides and Lactobacillus populations) and promoting the expression of lncRNA TUG1, thereby alleviating LPS-induced OP. |
| format | Article |
| id | doaj-art-575aee3bd32a4bf2be6fb6e367b92bf8 |
| institution | DOAJ |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-575aee3bd32a4bf2be6fb6e367b92bf82025-08-20T03:09:11ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-04-011510.3389/fcimb.2025.15356661535666Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expressionPengcheng Ma0Ruoyi Wang1Huizhi Chen2Jiachun Zheng3Weijie Yang4Bo Meng5Yifan Liu6Yao Lu7Jing Zhao8Hongwei Gao9Hongwei Gao10Shandong Public Health Clinical Center, Shandong University, Jinan, ChinaShandong Public Health Clinical Center, Shandong University, Jinan, ChinaShandong Public Health Clinical Center, Shandong University, Jinan, ChinaShandong Public Health Clinical Center, Shandong University, Jinan, ChinaShandong Public Health Clinical Center, Shandong University, Jinan, ChinaShandong Public Health Clinical Center, Shandong University, Jinan, ChinaShandong Public Health Clinical Center, Shandong University, Jinan, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaShandong Public Health Clinical Center, Shandong University, Jinan, ChinaSchool of Mechanical Engineering, Shandong University, Jinan, ChinaPurposeTo study whether fecal microbiota transplantation (FMT) can alleviate lipopolysaccharide (LPS)-induced osteoporosis (OP) by regulating the composition and abundance of gut microbiota and the expression level of long non-coding RNA (lncRNA) TUG1.MethodsTwenty C57BL/6 mice were selected. Two mice were randomly designated as fecal donors, while the remaining mice were randomly divided into control group, LPS group, and LPS + FMT group. Each group consisted of 6 mice. The mice in the LPS and LPS + FMT groups were intraperitoneally injected with LPS to establish the OP model, and the mice in the LPS + FMT group were treated with donor feces by gavage. Micro-CT was used to scan the femur specimens of mice, and the bone structural parameters of the control and LPS groups were compared to verify the effectiveness of the OP model. HE staining was used to compare the microstructure of femurs in the 3 groups. 16S rRNA gene sequencing was used to analyze the composition and abundance of gut microbiota in mice. Immunofluorescence staining was used to compare the expression levels of Runt-related transcription factor 2 (RUNX2) in the femur of the 3 groups. Real-time quantitative reverse transcription PCR (qRT-PCR) was used to compare the expression levels of lncRNA TUG1 in the intestines and serum of mice in the 3 groups.ResultsMicro-CT showed that compared with the control group, the mice in the LPS group had more bone loss. The bone mineral density, trabecular number, and trabecular thickness of the control group was higher, and the trabecular separation was smaller. The models were validated effectively. HE staining showed that compared with the control group, the bone trabeculae in the LPS group were thinner and sparse, while that in the LPS + FMT group were dense and clear. The 16s rRNA sequencing showed that the abundance of Bacteroides and Lactobacillus in LPS+FMT group was significantly higher than that in LPS group. Immunofluorescence staining showed that the RUNX2 level in the control group and LPS + FMT group was similar, and both were higher than that in the LPS group. The qRT-PCR results showed that the TUG1 mRNA level in the control group and LPS + FMT group was similar and significantly higher than that in the LPS group.ConclusionFMT can enhance osteoblast levels and improve bone structure by modulating the abundance of gut microbiota in OP mice (such as increasing Bacteroides and Lactobacillus populations) and promoting the expression of lncRNA TUG1, thereby alleviating LPS-induced OP.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1535666/fullfecal microbiota transplantationosteoporosisgut microbiotalncRNAlipopolysaccharides |
| spellingShingle | Pengcheng Ma Ruoyi Wang Huizhi Chen Jiachun Zheng Weijie Yang Bo Meng Yifan Liu Yao Lu Jing Zhao Hongwei Gao Hongwei Gao Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression Frontiers in Cellular and Infection Microbiology fecal microbiota transplantation osteoporosis gut microbiota lncRNA lipopolysaccharides |
| title | Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression |
| title_full | Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression |
| title_fullStr | Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression |
| title_full_unstemmed | Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression |
| title_short | Fecal microbiota transplantation alleviates lipopolysaccharide-induced osteoporosis by modulating gut microbiota and long non-coding RNA TUG1 expression |
| title_sort | fecal microbiota transplantation alleviates lipopolysaccharide induced osteoporosis by modulating gut microbiota and long non coding rna tug1 expression |
| topic | fecal microbiota transplantation osteoporosis gut microbiota lncRNA lipopolysaccharides |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1535666/full |
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