Protocol for the measurement of secreted acetylcholine in the lumen of mouse intestine using LC-MS

Protocol for the measurement of secreted acetylcholine in the lumen of mouse intestine using LC-MS

Summary: Acetylcholine (ACh) is a small-molecule neurotransmitter that regulates a wide range of neuronal and non-neuronal physiological functions. The non-neuronal roles of ACh are highly tissue specific, requiring precise measurements of its concentration in situ. Here, we present a protocol for m...

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Bibliographic Details
Main Authors: Evgenia Turtoi, Sylvain Henry, Charlène Joséphine, Marième Ndjim, Imène Gasmi, François Gerbe, Philippe Jay, Andrei Turtoi
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Immunology
Metabolomics
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002540
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http://www.sciencedirect.com/science/article/pii/S2666166725002540

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