Accurate cross-species 5mC detection for Oxford Nanopore sequencing in plants with DeepPlant

Abstract Nanopore sequencing enables comprehensive detection of 5-methylcytosine (5mC), particularly in repeat regions. However, CHH methylation detection in plants is limited by the scarcity of high-methylation positive samples, reducing generalization across species. Dorado, the only tool for plan...

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Bibliographic Details
Main Authors: He-Xu Chen, Zhen-Dong Liu, Xin Bai, Bo Wu, Rong Song, Hui-Cong Yao, Ying Chen, Wei Chi, Qian Hua, Liang Cheng, Chuan-Le Xiao
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-58576-x
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