Systematic Evaluation of Isolation Techniques and Freeze‐Thaw Effects on Plasma Extracellular Vesicle Heterogeneity and Subpopulation Profiling

Systematic Evaluation of Isolation Techniques and Freeze‐Thaw Effects on Plasma Extracellular Vesicle Heterogeneity and Subpopulation Profiling

ABSTRACT Extracellular vesicles (EVs) are increasingly recognized as promising disease biomarkers and therapeutic carriers. However, standardizing blood‐derived EV isolation remains challenging due to the heterogeneity of EV populations and variability among isolation techniques. In this study, we s...

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Bibliographic Details
Main Authors: Xueqi Li, Xiangyu Li, Lingjun Tong, Liqiao Hu, Yanfen Hong, Ruoyu Zhou, Zonghong Li, Ming Dong, Junjie Hou, Tao Xu, Wen Zhong
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Journal of Extracellular Biology
Subjects:
extracellular vesicles
EV isolation
plasma
proteomics
small EVs
single EV
Online Access:https://doi.org/10.1002/jex2.70058
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https://doi.org/10.1002/jex2.70058

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