Protocol to derive postganglionic parasympathetic neurons using human pluripotent stem cells for electrophysiological and functional assessment

Summary: Accessing untransformed human primary parasympathetic neurons is extremely challenging due to their inaccessibility in patients. Therefore, a human pluripotent stem cell-based approach to generate pure parasympathetic neurons is valuable for studying their development, maturation, and patho...

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Bibliographic Details
Main Authors: Christina James, Hsueh-Fu Wu, Jennifer Art, Archie Patel, Nadja Zeltner
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004071
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