In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin
Traditional Chinese medicine (TCM), renowned for environmentally friendly and natural characteristics, has emerged as a promising alternative to conventional antibiotics. This study evaluated the antibacterial efficacy of six TCM-derived monomers (quercetin, luteolin, pyrogallol, gallic acid, tannic...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006318 |
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| author | Wenhao Wu Jiahao Zhang Xiaoyin He Wei Li Chun Liu Biao Jiang Youlu Su |
| author_facet | Wenhao Wu Jiahao Zhang Xiaoyin He Wei Li Chun Liu Biao Jiang Youlu Su |
| author_sort | Wenhao Wu |
| collection | DOAJ |
| description | Traditional Chinese medicine (TCM), renowned for environmentally friendly and natural characteristics, has emerged as a promising alternative to conventional antibiotics. This study evaluated the antibacterial efficacy of six TCM-derived monomers (quercetin, luteolin, pyrogallol, gallic acid, tannic acid, and L-epicatechin) from Pithecellobium clypearia against 11 stains of aquatic pathogens, including a drug-resistant Aeromonas schubertii strains. Employing the Oxford cup diffusion and micro broth dilution methods, we discovered these components demonstrated varying degrees of antibacterial activity against the tested pathogens. Pyrogallol, luteolin and tannic acid exhibiting particularly remarkable performance. with pyrogallol exhibiting an impressive minimum inhibitory concentration (MIC) of just 15.63 µg/mL, and luteolin and tannic acid ranged from 62.50 to 500 µg/mL. These compounds demonstrate greater efficacy against the multi-drug resistant Aeromonas schubertii strain WL23R compared to the sensitive A. schubertii strain WL23S, indicating their potential in combating antibiotic resistance. Additionally, the combination of pyrogallol, luteolin, and tannic synergy with enrofloxacin (ENR) enhanced the potency of existing antimicrobials and potentially reducing dosage needs. These results provided evidence that the six monomers, particularly pyrogallol, luteolin, and tannic acid, exhibited substantial antibacterial properties with potential applications as antibiotic alternatives in aquaculture. |
| format | Article |
| id | doaj-art-55020ff0fdd04860a56918f1fe271ad7 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-55020ff0fdd04860a56918f1fe271ad72025-02-06T05:12:05ZengElsevierAquaculture Reports2352-51342025-03-0140102543In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacinWenhao Wu0Jiahao Zhang1Xiaoyin He2Wei Li3Chun Liu4Biao Jiang5Youlu Su6Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, ChinaGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, ChinaGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, ChinaGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, ChinaGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, ChinaCorresponding authors.; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, ChinaCorresponding authors.; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, ChinaTraditional Chinese medicine (TCM), renowned for environmentally friendly and natural characteristics, has emerged as a promising alternative to conventional antibiotics. This study evaluated the antibacterial efficacy of six TCM-derived monomers (quercetin, luteolin, pyrogallol, gallic acid, tannic acid, and L-epicatechin) from Pithecellobium clypearia against 11 stains of aquatic pathogens, including a drug-resistant Aeromonas schubertii strains. Employing the Oxford cup diffusion and micro broth dilution methods, we discovered these components demonstrated varying degrees of antibacterial activity against the tested pathogens. Pyrogallol, luteolin and tannic acid exhibiting particularly remarkable performance. with pyrogallol exhibiting an impressive minimum inhibitory concentration (MIC) of just 15.63 µg/mL, and luteolin and tannic acid ranged from 62.50 to 500 µg/mL. These compounds demonstrate greater efficacy against the multi-drug resistant Aeromonas schubertii strain WL23R compared to the sensitive A. schubertii strain WL23S, indicating their potential in combating antibiotic resistance. Additionally, the combination of pyrogallol, luteolin, and tannic synergy with enrofloxacin (ENR) enhanced the potency of existing antimicrobials and potentially reducing dosage needs. These results provided evidence that the six monomers, particularly pyrogallol, luteolin, and tannic acid, exhibited substantial antibacterial properties with potential applications as antibiotic alternatives in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513424006318Pithecellobium clypeariaMonomerAquatic pathogensAntibacterial activitySynergy |
| spellingShingle | Wenhao Wu Jiahao Zhang Xiaoyin He Wei Li Chun Liu Biao Jiang Youlu Su In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin Aquaculture Reports Pithecellobium clypearia Monomer Aquatic pathogens Antibacterial activity Synergy |
| title | In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin |
| title_full | In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin |
| title_fullStr | In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin |
| title_full_unstemmed | In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin |
| title_short | In vitro evaluation of the antibacterial effects of monomer components of Pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin |
| title_sort | in vitro evaluation of the antibacterial effects of monomer components of pithecellobium clypearia against aquatic pathogens and synergy with enrofloxacin |
| topic | Pithecellobium clypearia Monomer Aquatic pathogens Antibacterial activity Synergy |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006318 |
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