Characterization and structural analysis of a leucine aminopeptidase using site-directed mutagenesis

Characterization and structural analysis of a leucine aminopeptidase using site-directed mutagenesis

Abstract Amp0279 (EC 3.4.11.24, GenBank: CP000817.1) is a Co2+-dependent leucine aminopeptidase from the Lysinibacillus sphaericus C3-41 genome. After analyses using molecular docking and spatial structure analysis, site-directed mutagenesis mutants were performed as Amp0279-R131E, Amp0279-R131H, Am...

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Bibliographic Details
Main Authors: Yuqi Men, Yang Liu, Dongjie Yin, Guan Wang, Rui Qin, Hairong Xiong, Yawei Wang
Format: Article
Language:English
Published: SpringerOpen 2024-12-01
Series:AMB Express
Subjects:
Aminopeptidase
Site-directed mutagenesis
pH shift
Molecular docking
Hydrogen bond
π-Cation interaction
Online Access:https://doi.org/10.1186/s13568-024-01793-2
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https://doi.org/10.1186/s13568-024-01793-2

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