Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed
This study aimed to evaluate the extraction, semi-purification, encapsulation, and release of proteolytic enzymes from the hepatopancreas of white shrimp (Litopenaeus vannamei) for potential inclusion in pelleted feed for tilapia. Proteases were extracted using hydrophobic interaction chromatography...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X25000097 |
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| author | Pablo Sergio Osuna-Amarillas Kenia Yamileth Medina-Rivera Martha Elisa Rivas-Vega Francisco Javier Cinco-Moroyoqui Octavio Cota-Arriola Wilfrido Torres-Arriola Norma Aurora Stephens-Camacho Anselmo Miranda-Baeza |
| author_facet | Pablo Sergio Osuna-Amarillas Kenia Yamileth Medina-Rivera Martha Elisa Rivas-Vega Francisco Javier Cinco-Moroyoqui Octavio Cota-Arriola Wilfrido Torres-Arriola Norma Aurora Stephens-Camacho Anselmo Miranda-Baeza |
| author_sort | Pablo Sergio Osuna-Amarillas |
| collection | DOAJ |
| description | This study aimed to evaluate the extraction, semi-purification, encapsulation, and release of proteolytic enzymes from the hepatopancreas of white shrimp (Litopenaeus vannamei) for potential inclusion in pelleted feed for tilapia. Proteases were extracted using hydrophobic interaction chromatography, yielding a semi-purified shrimp hepatopancreas extract (SPESH). SDS-PAGE analysis identified four distinct protein bands and the optimal conditions for enzyme activity were determined across a range of temperatures (0–100 °C) and pH levels (4–11). Inhibition assays revealed the presence of serine proteases, primarily trypsin-type enzymes, alongside aspartic and cysteine proteases. SPESH exhibited maximum proteolytic activity at 40 °C and pH 9. Proteolytic activity from the sodium alginate capsules was observed 30 min after encapsulation release testing, with a stable trend up to 120 min. Over the 0–16 days storage period, the proteolytic activity of capsules incubated for 60 min (36.3 ± 2.80 to 38.4 ± 1.21 AU) and 120 min (45.4 ± 1.40 to 47.8 ± 0.28 AU) prevailed stable with no significant differences (P < 0.05), indicating consistent enzyme release. The encapsulation method allowed a gradual release of enzymes at pH conditions representative of the Nile tilapia (Oreochromis niloticus) digestive tract, supporting its potential for controlled delivery and enzyme supplementation in feed. |
| format | Article |
| id | doaj-art-5351b0eae80f47c0891c324e4d8edc34 |
| institution | DOAJ |
| issn | 2772-753X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj-art-5351b0eae80f47c0891c324e4d8edc342025-08-20T02:52:20ZengElsevierFood Chemistry Advances2772-753X2025-03-01610089310.1016/j.focha.2025.100893Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feedPablo Sergio Osuna-Amarillas0Kenia Yamileth Medina-Rivera1Martha Elisa Rivas-Vega2Francisco Javier Cinco-Moroyoqui3Octavio Cota-Arriola4Wilfrido Torres-Arriola5Norma Aurora Stephens-Camacho6Anselmo Miranda-Baeza7Laboratorio de Tecnologías de cultivo de organismos acuáticos. Universidad Estatal de Sonora (UES), Navojoa, Sonora, 85875, MexicoMaestría en Producción y Gestión de Negocios Agropecuarios. Universidad Estatal de Sonora (UES), Navojoa, Sonora, 85875, MexicoLaboratorio de Tecnologías de cultivo de organismos acuáticos. Universidad Estatal de Sonora (UES), Navojoa, Sonora, 85875, MexicoDepartamento de Investigación y Posgrado en Alimentos (DIPA). Universidad de Sonora (UNISON), Hermosillo, Sonora, 83000, MexicoIngeniería Ambiental, Unidad Académica Hermosillo. Universidad Estatal de Sonora (UES). Hermosillo, Sonora, 83100, MexicoDepartamento de Investigación y Posgrado en Alimentos (DIPA). Universidad de Sonora (UNISON), Hermosillo, Sonora, 83000, MexicoNutrición Humana, Universidad Estatal de Sonora (UES). Navojoa, Sonora, 85875, MexicoLaboratorio de Tecnologías de cultivo de organismos acuáticos. Universidad Estatal de Sonora (UES), Navojoa, Sonora, 85875, Mexico; Corresponding author at: Laboratorio de Tecnologías de cultivo de organismos acuáticos. Universidad Estatal de Sonora (UES), Navojoa, Sonora, 85875, México.This study aimed to evaluate the extraction, semi-purification, encapsulation, and release of proteolytic enzymes from the hepatopancreas of white shrimp (Litopenaeus vannamei) for potential inclusion in pelleted feed for tilapia. Proteases were extracted using hydrophobic interaction chromatography, yielding a semi-purified shrimp hepatopancreas extract (SPESH). SDS-PAGE analysis identified four distinct protein bands and the optimal conditions for enzyme activity were determined across a range of temperatures (0–100 °C) and pH levels (4–11). Inhibition assays revealed the presence of serine proteases, primarily trypsin-type enzymes, alongside aspartic and cysteine proteases. SPESH exhibited maximum proteolytic activity at 40 °C and pH 9. Proteolytic activity from the sodium alginate capsules was observed 30 min after encapsulation release testing, with a stable trend up to 120 min. Over the 0–16 days storage period, the proteolytic activity of capsules incubated for 60 min (36.3 ± 2.80 to 38.4 ± 1.21 AU) and 120 min (45.4 ± 1.40 to 47.8 ± 0.28 AU) prevailed stable with no significant differences (P < 0.05), indicating consistent enzyme release. The encapsulation method allowed a gradual release of enzymes at pH conditions representative of the Nile tilapia (Oreochromis niloticus) digestive tract, supporting its potential for controlled delivery and enzyme supplementation in feed.http://www.sciencedirect.com/science/article/pii/S2772753X25000097Enzyme encapsulationDigestive proteasesProteolytic activitySodium alginate capsulesShrimp waste |
| spellingShingle | Pablo Sergio Osuna-Amarillas Kenia Yamileth Medina-Rivera Martha Elisa Rivas-Vega Francisco Javier Cinco-Moroyoqui Octavio Cota-Arriola Wilfrido Torres-Arriola Norma Aurora Stephens-Camacho Anselmo Miranda-Baeza Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed Food Chemistry Advances Enzyme encapsulation Digestive proteases Proteolytic activity Sodium alginate capsules Shrimp waste |
| title | Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed |
| title_full | Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed |
| title_fullStr | Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed |
| title_full_unstemmed | Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed |
| title_short | Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed |
| title_sort | evaluation of encapsulated proteolytic enzymes from white shrimp litopenaeus vannamei hepatopancreas for potential supplementation in tilapia feed |
| topic | Enzyme encapsulation Digestive proteases Proteolytic activity Sodium alginate capsules Shrimp waste |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X25000097 |
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