Evaluation of encapsulated proteolytic enzymes from white shrimp (Litopenaeus vannamei) hepatopancreas for potential supplementation in tilapia feed
This study aimed to evaluate the extraction, semi-purification, encapsulation, and release of proteolytic enzymes from the hepatopancreas of white shrimp (Litopenaeus vannamei) for potential inclusion in pelleted feed for tilapia. Proteases were extracted using hydrophobic interaction chromatography...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | Food Chemistry Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X25000097 |
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| Summary: | This study aimed to evaluate the extraction, semi-purification, encapsulation, and release of proteolytic enzymes from the hepatopancreas of white shrimp (Litopenaeus vannamei) for potential inclusion in pelleted feed for tilapia. Proteases were extracted using hydrophobic interaction chromatography, yielding a semi-purified shrimp hepatopancreas extract (SPESH). SDS-PAGE analysis identified four distinct protein bands and the optimal conditions for enzyme activity were determined across a range of temperatures (0–100 °C) and pH levels (4–11). Inhibition assays revealed the presence of serine proteases, primarily trypsin-type enzymes, alongside aspartic and cysteine proteases. SPESH exhibited maximum proteolytic activity at 40 °C and pH 9. Proteolytic activity from the sodium alginate capsules was observed 30 min after encapsulation release testing, with a stable trend up to 120 min. Over the 0–16 days storage period, the proteolytic activity of capsules incubated for 60 min (36.3 ± 2.80 to 38.4 ± 1.21 AU) and 120 min (45.4 ± 1.40 to 47.8 ± 0.28 AU) prevailed stable with no significant differences (P < 0.05), indicating consistent enzyme release. The encapsulation method allowed a gradual release of enzymes at pH conditions representative of the Nile tilapia (Oreochromis niloticus) digestive tract, supporting its potential for controlled delivery and enzyme supplementation in feed. |
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| ISSN: | 2772-753X |