The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytes
ABSTRACT Follicular fluid is a haven that provides a microenvironment for the development and maturation of oocytes. It is a medium that provides a means of communication within the follicular cells. The role of follicular fluid in the development and maturation of follicles and oocytes involves the...
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Universidade Federal de Minas Gerais
2025-01-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352025000100106&lng=en&tlng=en |
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| author | A.A. Ammari AR. Alhimaidi K.N. Yaseen B.O. Almutairi R.A. Amran A. AL-juaimlan A. Rady |
| author_facet | A.A. Ammari AR. Alhimaidi K.N. Yaseen B.O. Almutairi R.A. Amran A. AL-juaimlan A. Rady |
| author_sort | A.A. Ammari |
| collection | DOAJ |
| description | ABSTRACT Follicular fluid is a haven that provides a microenvironment for the development and maturation of oocytes. It is a medium that provides a means of communication within the follicular cells. The role of follicular fluid in the development and maturation of follicles and oocytes involves the actions of certain genes. Here, we examined the impact of CFF on the gene expression of camel oocytes during (IVM). Hypothetically, IVM medium was enhanced with 2.5% CFF, and we analyzed the expression of several genes including Bax, Bcl-2, P53, Acta2, TGFβ, Cx43, and Tagln. Consequently, the follicular fluid from camels had a substantial effect on the mRNA transcript level of the BCL2 gene, which is related to apoptosis. Acta2 and Tagln genes that relate to cytoskeletal protein “actin” in transforming growth factor beta (TGFβ) pathway were also modulated. However, the follicular fluid increased the expression of TGFβ in camel oocytes. This study showcases the beneficial effects of CFF on camel oocyte Gene Expression and provides a platform for studying the many mechanisms involved in oocyte meiosis, particularly those associated with the TGFβ pathway. We recommend using higher dosage and measuring the expression levels of certain genes involved in programmed cell death and inflammation. |
| format | Article |
| id | doaj-art-52e20fdc50eb4911b5834459c1a1e3a7 |
| institution | Kabale University |
| issn | 1678-4162 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj-art-52e20fdc50eb4911b5834459c1a1e3a72025-01-21T07:37:52ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622025-01-0177110.1590/1678-4162-13289The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytesA.A. Ammarihttps://orcid.org/0000-0002-1900-8446AR. Alhimaidihttps://orcid.org/0000-0002-8608-334XK.N. Yaseenhttps://orcid.org/0009-0001-0437-3918B.O. Almutairihttps://orcid.org/0000-0002-4697-5204R.A. Amranhttps://orcid.org/0000-0002-8612-7576A. AL-juaimlanhttps://orcid.org/0009-0009-1785-5383A. Radyhttps://orcid.org/0000-0001-5554-5858ABSTRACT Follicular fluid is a haven that provides a microenvironment for the development and maturation of oocytes. It is a medium that provides a means of communication within the follicular cells. The role of follicular fluid in the development and maturation of follicles and oocytes involves the actions of certain genes. Here, we examined the impact of CFF on the gene expression of camel oocytes during (IVM). Hypothetically, IVM medium was enhanced with 2.5% CFF, and we analyzed the expression of several genes including Bax, Bcl-2, P53, Acta2, TGFβ, Cx43, and Tagln. Consequently, the follicular fluid from camels had a substantial effect on the mRNA transcript level of the BCL2 gene, which is related to apoptosis. Acta2 and Tagln genes that relate to cytoskeletal protein “actin” in transforming growth factor beta (TGFβ) pathway were also modulated. However, the follicular fluid increased the expression of TGFβ in camel oocytes. This study showcases the beneficial effects of CFF on camel oocyte Gene Expression and provides a platform for studying the many mechanisms involved in oocyte meiosis, particularly those associated with the TGFβ pathway. We recommend using higher dosage and measuring the expression levels of certain genes involved in programmed cell death and inflammation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352025000100106&lng=en&tlng=encamel oocytegene expressionin vitro maturationfollicular fluid |
| spellingShingle | A.A. Ammari AR. Alhimaidi K.N. Yaseen B.O. Almutairi R.A. Amran A. AL-juaimlan A. Rady The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytes Arquivo Brasileiro de Medicina Veterinária e Zootecnia camel oocyte gene expression in vitro maturation follicular fluid |
| title | The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytes |
| title_full | The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytes |
| title_fullStr | The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytes |
| title_full_unstemmed | The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytes |
| title_short | The influence of follicular fluid on the regulation of apoptosis and related genes during IVM of camel oocytes |
| title_sort | influence of follicular fluid on the regulation of apoptosis and related genes during ivm of camel oocytes |
| topic | camel oocyte gene expression in vitro maturation follicular fluid |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352025000100106&lng=en&tlng=en |
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