Production of Multifunctional Hydrolysates from the <i>Lupinus mutabilis</i> Protein Using a <i>Micrococcus</i> sp. PC7 Protease

Production of Multifunctional Hydrolysates from the <i>Lupinus mutabilis</i> Protein Using a <i>Micrococcus</i> sp. PC7 Protease

The growing demand for functional foods has driven the search for bioactive compounds derived from plant proteins. <i>Lupinus mutabilis</i> “Tarwi”, a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bioactive peptides (BPs). In th...

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Bibliographic Details
Main Authors: Keyla Sofía Llontop-Bernabé, Arturo Intiquilla, Carlos Ramirez-Veliz, Marco Santos, Karim Jiménez-Aliaga, Amparo Iris Zavaleta, Samuel Paterson, Blanca Hernández-Ledesma
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:BioTech
Subjects:
<i>Lupinus mutabilis</i>
albumin fraction
ultrafiltration
protease PC7
multifunctionality
Online Access:https://www.mdpi.com/2673-6284/14/2/32
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Summary:The growing demand for functional foods has driven the search for bioactive compounds derived from plant proteins. <i>Lupinus mutabilis</i> “Tarwi”, a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bioactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functionally classified into three groups: cellular component (35.57%), molecular function (33.45%), and biological process (30.97%). The isolated AF was hydrolysed with the native protease PC7 (HAP), optimizing the E/S ratio and time parameters. Additionally, sequential hydrolysis of the PC7 protease and alcalase (HAPA) was performed. In vitro multifunctionality assays, HAP and HAPA demonstrated the ability to scavenge radicals (ABTS and ORAC) and inhibit angiotensin-converting enzyme (ACE)-I and dipeptidyl peptidase IV (DPP-IV). The findings of this study highlight the potential of <i>L. mutabilis</i> albumin hydrolysate as a multifunctional ingredient for functional foods aimed at managing chronic conditions associated with oxidative stress, hypertension, and/or metabolic disorders.
ISSN:2673-6284

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