Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD
So-Cheong-Ryong-Tang, which is a standardized Korean medicine of the National Health Insurance, is a traditional prescription for the treatment of allergic rhinitis, bronchitis, and bronchial asthma. Simultaneous analysis and development of SCRT is essential for its stability, efficacy, and risk man...
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| Format: | Article |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/2875681 |
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| author | Ji Hyun Jeong Seon Yu Lee Bo Na Kim Guk Yeo Lee Seong Ho Ham |
| author_facet | Ji Hyun Jeong Seon Yu Lee Bo Na Kim Guk Yeo Lee Seong Ho Ham |
| author_sort | Ji Hyun Jeong |
| collection | DOAJ |
| description | So-Cheong-Ryong-Tang, which is a standardized Korean medicine of the National Health Insurance, is a traditional prescription for the treatment of allergic rhinitis, bronchitis, and bronchial asthma. Simultaneous analysis and development of SCRT is essential for its stability, efficacy, and risk management. In this study, a simple, reliable, and accurate method using ultrahigh-performance liquid chromatography (UPLC) fingerprinting with a diode array detector (DAD) was developed for the simultaneous analysis. The chromatographic separation of the analytes was performed by an ACQUITY UPLC BEH C18 column (1.7 μM, 2.1 × 100 mm, Waters) with a mobile phase of water containing 0.01% (v/v) phosphoric acid and acetonitrile containing 0.01% (v/v) phosphoric acid. The flow rate and detection wavelength were set at 0.4 mL/min and 215, 230, 254, and 280 nm. All calibration curves of the thirteen components showed good linearity (R2 > 0.999). The limit of detection and limit of quantification ranged 0.001–0.360 and 0.004–1.200 µg/mL, respectively. The relative standard deviation (RSD) of intra- and interday was less than 2.60%, and the recoveries were within the range 76.08–103.79% with an RSD value of 0.03–1.50%. The results showed that the developed method was simple, reliable, accurate, sensitive, and precise for the quantification of bioactive components of SCRT. |
| format | Article |
| id | doaj-art-51e79565475a4c1889e7eed612c95019 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-51e79565475a4c1889e7eed612c950192025-02-03T01:20:15ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/28756812875681Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DADJi Hyun Jeong0Seon Yu Lee1Bo Na Kim2Guk Yeo Lee3Seong Ho Ham4National Development Institute of Korean Medicine, Udae land gil 288, Jangheung-gun, Jeollanam-do 59338, Republic of KoreaNational Development Institute of Korean Medicine, Udae land gil 288, Jangheung-gun, Jeollanam-do 59338, Republic of KoreaNational Development Institute of Korean Medicine, Udae land gil 288, Jangheung-gun, Jeollanam-do 59338, Republic of KoreaNational Development Institute of Korean Medicine, Udae land gil 288, Jangheung-gun, Jeollanam-do 59338, Republic of KoreaNational Development Institute of Korean Medicine, Udae land gil 288, Jangheung-gun, Jeollanam-do 59338, Republic of KoreaSo-Cheong-Ryong-Tang, which is a standardized Korean medicine of the National Health Insurance, is a traditional prescription for the treatment of allergic rhinitis, bronchitis, and bronchial asthma. Simultaneous analysis and development of SCRT is essential for its stability, efficacy, and risk management. In this study, a simple, reliable, and accurate method using ultrahigh-performance liquid chromatography (UPLC) fingerprinting with a diode array detector (DAD) was developed for the simultaneous analysis. The chromatographic separation of the analytes was performed by an ACQUITY UPLC BEH C18 column (1.7 μM, 2.1 × 100 mm, Waters) with a mobile phase of water containing 0.01% (v/v) phosphoric acid and acetonitrile containing 0.01% (v/v) phosphoric acid. The flow rate and detection wavelength were set at 0.4 mL/min and 215, 230, 254, and 280 nm. All calibration curves of the thirteen components showed good linearity (R2 > 0.999). The limit of detection and limit of quantification ranged 0.001–0.360 and 0.004–1.200 µg/mL, respectively. The relative standard deviation (RSD) of intra- and interday was less than 2.60%, and the recoveries were within the range 76.08–103.79% with an RSD value of 0.03–1.50%. The results showed that the developed method was simple, reliable, accurate, sensitive, and precise for the quantification of bioactive components of SCRT.http://dx.doi.org/10.1155/2018/2875681 |
| spellingShingle | Ji Hyun Jeong Seon Yu Lee Bo Na Kim Guk Yeo Lee Seong Ho Ham Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD Journal of Analytical Methods in Chemistry |
| title | Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD |
| title_full | Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD |
| title_fullStr | Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD |
| title_full_unstemmed | Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD |
| title_short | Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD |
| title_sort | development of simultaneous analysis of thirteen bioactive compounds in so cheong ryong tang using uplc dad |
| url | http://dx.doi.org/10.1155/2018/2875681 |
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