Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products
Milk and its products can be frequently contaminated with enterotoxigenic and methicillin-resistant S. aureus, and in such a case, it causes various diseases, especially staphylococcal food poisoning. In the present study, 100 sample materials (50 goat milk, 25 goat cream, and 25 goat cheese) were c...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kyrgyz Turkish Manas University
2022-12-01
|
| Series: | MANAS: Journal of Engineering |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/2693093 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832542953584721920 |
|---|---|
| author | Mustafa Bilgin Mustafa Atasever |
| author_facet | Mustafa Bilgin Mustafa Atasever |
| author_sort | Mustafa Bilgin |
| collection | DOAJ |
| description | Milk and its products can be frequently contaminated with enterotoxigenic and methicillin-resistant S. aureus, and in such a case, it causes various diseases, especially staphylococcal food poisoning. In the present study, 100 sample materials (50 goat milk, 25 goat cream, and 25 goat cheese) were collected from 65 livestock farms in Erzurum. All samples were analyzed and tested selectively according to the EN ISO 6888-1 procedure standard. The obtained isolates were examined with the PCR in terms of nuc, Panton-Valentine Leukocidin (PVL), mecA, and enterotoxin genes. S. aureus was detected in 4 of 50 (8%) milk samples, 2 of 25 (8%) cream samples, 3 of 25 (12%) cheese samples, and 9 of the total samples (9%). While the incidence of methicillin resistance was found to be 3% by the disc diffusion method, the incidence of the mecA gene was found to be 2% by PCR. In terms of enterotoxin genes, 8% (4/50) of milk samples, 12% (3/25) of cheese samples, and 8% (2/25) of cream samples contained at least one enterotoxin gene. In total, 9% of 100 samples contained enterotoxigenic S. aureus. In conclusion, the consumption of enterotoxigenic and mecA positive S. aureus-containing raw milk, dairy products that have not been adequately heat-treated, or milk and products that are not kept in proper storage conditions pose a danger to public health. |
| format | Article |
| id | doaj-art-4f55e2872f4b4dbcb8a49c5a3e88f84a |
| institution | Kabale University |
| issn | 1694-7398 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Kyrgyz Turkish Manas University |
| record_format | Article |
| series | MANAS: Journal of Engineering |
| spelling | doaj-art-4f55e2872f4b4dbcb8a49c5a3e88f84a2025-02-03T12:02:41ZengKyrgyz Turkish Manas UniversityMANAS: Journal of Engineering1694-73982022-12-0110215115810.51354/mjen.11853461437Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its productsMustafa Bilgin0https://orcid.org/0000-0001-9212-7823Mustafa Atasever1https://orcid.org/0000-0002-1627-5565Atatürk ÜnicersitesiATATÜRK ÜNİVERSİTESİMilk and its products can be frequently contaminated with enterotoxigenic and methicillin-resistant S. aureus, and in such a case, it causes various diseases, especially staphylococcal food poisoning. In the present study, 100 sample materials (50 goat milk, 25 goat cream, and 25 goat cheese) were collected from 65 livestock farms in Erzurum. All samples were analyzed and tested selectively according to the EN ISO 6888-1 procedure standard. The obtained isolates were examined with the PCR in terms of nuc, Panton-Valentine Leukocidin (PVL), mecA, and enterotoxin genes. S. aureus was detected in 4 of 50 (8%) milk samples, 2 of 25 (8%) cream samples, 3 of 25 (12%) cheese samples, and 9 of the total samples (9%). While the incidence of methicillin resistance was found to be 3% by the disc diffusion method, the incidence of the mecA gene was found to be 2% by PCR. In terms of enterotoxin genes, 8% (4/50) of milk samples, 12% (3/25) of cheese samples, and 8% (2/25) of cream samples contained at least one enterotoxin gene. In total, 9% of 100 samples contained enterotoxigenic S. aureus. In conclusion, the consumption of enterotoxigenic and mecA positive S. aureus-containing raw milk, dairy products that have not been adequately heat-treated, or milk and products that are not kept in proper storage conditions pose a danger to public health.https://dergipark.org.tr/en/download/article-file/2693093goat milkmethicillin resistancestaphylococcal enterotoxinsstaphylococcus aureus |
| spellingShingle | Mustafa Bilgin Mustafa Atasever Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products MANAS: Journal of Engineering goat milk methicillin resistance staphylococcal enterotoxins staphylococcus aureus |
| title | Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products |
| title_full | Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products |
| title_fullStr | Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products |
| title_full_unstemmed | Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products |
| title_short | Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products |
| title_sort | determining the enterotoxin genes and methicillin resistance in staphylococcus aureus isolated from goat milk and its products |
| topic | goat milk methicillin resistance staphylococcal enterotoxins staphylococcus aureus |
| url | https://dergipark.org.tr/en/download/article-file/2693093 |
| work_keys_str_mv | AT mustafabilgin determiningtheenterotoxingenesandmethicillinresistanceinstaphylococcusaureusisolatedfromgoatmilkanditsproducts AT mustafaatasever determiningtheenterotoxingenesandmethicillinresistanceinstaphylococcusaureusisolatedfromgoatmilkanditsproducts |