Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung
We compared lung delivery methods of recombinant adenovirus (rAd): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined us...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Pulmonary Medicine |
| Online Access: | http://dx.doi.org/10.1155/2011/918036 |
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| author | Jeffrey A. Kazzaz Marlene S. Strayer Jichuan Wu Daniel J. Malone Hshi-chi Koo Thomas H. Shaffer Jonathan M. Davis David S. Strayer Marla R. Wolfson |
| author_facet | Jeffrey A. Kazzaz Marlene S. Strayer Jichuan Wu Daniel J. Malone Hshi-chi Koo Thomas H. Shaffer Jonathan M. Davis David S. Strayer Marla R. Wolfson |
| author_sort | Jeffrey A. Kazzaz |
| collection | DOAJ |
| description | We compared lung delivery methods of recombinant adenovirus (rAd): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2–4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy. |
| format | Article |
| id | doaj-art-4f3fc23b5ca449889e77e4a27e8126e1 |
| institution | DOAJ |
| issn | 2090-1836 2090-1844 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pulmonary Medicine |
| spelling | doaj-art-4f3fc23b5ca449889e77e4a27e8126e12025-08-20T03:20:17ZengWileyPulmonary Medicine2090-18362090-18442011-01-01201110.1155/2011/918036918036Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal LungJeffrey A. Kazzaz0Marlene S. Strayer1Jichuan Wu2Daniel J. Malone3Hshi-chi Koo4Thomas H. Shaffer5Jonathan M. Davis6David S. Strayer7Marla R. Wolfson8The Cardiopulmonary Research Institute and Departments of Medicine and Pediatrics, SUNY Stony Brook School of Medicine, Winthrop University Hospital, Mineola, NY 11507, USADepartment of Pathology, Thomas Jefferson University Medical Center, Philadelphia, PA 19107, USADepartments of Physiology and Pediatrics, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USADepartments of Physiology and Pediatrics, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USAThe Cardiopulmonary Research Institute and Departments of Medicine and Pediatrics, SUNY Stony Brook School of Medicine, Winthrop University Hospital, Mineola, NY 11507, USADepartments of Physiology and Pediatrics, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USAThe Cardiopulmonary Research Institute and Departments of Medicine and Pediatrics, SUNY Stony Brook School of Medicine, Winthrop University Hospital, Mineola, NY 11507, USADepartment of Pathology, Thomas Jefferson University Medical Center, Philadelphia, PA 19107, USADepartments of Physiology and Pediatrics, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USAWe compared lung delivery methods of recombinant adenovirus (rAd): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2–4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.http://dx.doi.org/10.1155/2011/918036 |
| spellingShingle | Jeffrey A. Kazzaz Marlene S. Strayer Jichuan Wu Daniel J. Malone Hshi-chi Koo Thomas H. Shaffer Jonathan M. Davis David S. Strayer Marla R. Wolfson Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung Pulmonary Medicine |
| title | Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung |
| title_full | Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung |
| title_fullStr | Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung |
| title_full_unstemmed | Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung |
| title_short | Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung |
| title_sort | perfluorochemical liquid adenovirus suspensions enhance gene delivery to the distal lung |
| url | http://dx.doi.org/10.1155/2011/918036 |
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