Use of Green Fluorescent Protein/Flp Recombinase Fusion Protein and Flow Cytometric Sorting to Enrich for Cells Undergoing Flp-Mediated Recombination
Flp recombinase has been used extensively for in vivo manipulation of eukaryotic DNA at specific sequences designated as FRT sites. We developed a method to use Flp-mediated recombination without the need for drug resistance or metabolic selection of cells in which recombination has occurred. We gen...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00285rr02 |
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