Analysis of Digoxin and Metildigoxin in Whole Blood Using Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry

A simple and rapid UPLC/MS/MS method has been developed and validated for the analysis of digoxin and metildigoxin in whole blood. Samples were prepared by SPE extraction with Oasis HLB columns. Separation was achieved with an ACQUITY UPLC HSS T3 column (2.1×100; 1.8 μm), at 35°C. The mobile phase c...

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Bibliographic Details
Main Authors: Paula Melo, Rita Machado, Helena M. Teixeira
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2012/975824
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