Improved Extraction Methods to Isolate High Molecular Weight DNA From Magnaporthaceae and Other Grass Root Fungi for Long-Read Whole Genome Sequencing

Improved Extraction Methods to Isolate High Molecular Weight DNA From Magnaporthaceae and Other Grass Root Fungi for Long-Read Whole Genome Sequencing

This manuscript details two modified protocols for the isolation of long-stranded or high molecular weight (HMW) DNA from Magnaporthaceae (Ascomycota) fungal mycelium intended for whole genome sequencing. The Cytiva Nucleon PhytoPure and the Macherey-Nagel NucleoBond HMW DNA kits were selected becau...

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Main Authors: Michelle Grey, Jackie Freeman, Jason Rudd, Naomi Irish, Gail Canning, Tania Chancellor, Javier Palma-Guerrero, Rowena Hill, Neil Hall, Kim Hammond-Kosack, Mark McMullan
Format: Article
Language:English
Published: Bio-protocol LLC 2025-03-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5245&type=0
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