High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic Elements
The impact of antibiotic therapy on the spread of antibiotic resistance genes (ARGs) and its relationship to gut microbiota remains unclear. This study investigated changes in ARGs, mobile genetic elements (MGEs), and gut microbial composition following tilmicosin administration in pigs. Thirty pigs...
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2024-12-01
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| author | Tao Chen Minxing Zhao Majian Chen Xiaoyue Tang Yuliang Qian Xiaoting Li Yan Wang Xindi Liao Yinbao Wu |
| author_facet | Tao Chen Minxing Zhao Majian Chen Xiaoyue Tang Yuliang Qian Xiaoting Li Yan Wang Xindi Liao Yinbao Wu |
| author_sort | Tao Chen |
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| description | The impact of antibiotic therapy on the spread of antibiotic resistance genes (ARGs) and its relationship to gut microbiota remains unclear. This study investigated changes in ARGs, mobile genetic elements (MGEs), and gut microbial composition following tilmicosin administration in pigs. Thirty pigs were randomly divided into control (CK), low-concentration (0.2 g/kg; L), and high-concentration (0.4 g/kg; H) groups. Tilmicosin concentration in manure peaked on day 16 of dosing and dropped below detectable levels by day 13 of the withdrawal period. While tilmicosin did not significantly affect the total abundance of macrolide resistance genes (MRGs) (<i>p</i> > 0.05), it significantly increased the abundance of the multidrug resistance gene <i>tol</i>C in the H group compared with the L and CK groups during the withdrawal period (<i>p</i> < 0.05). This increase was associated with a coincidental rise in the abundance of MGEs (e.g., <i>int</i>1 and <i>int</i>2) and the growth of potential <i>tol</i>C-hosting bacteria such as <i>Paenalcaligenes</i> and <i>Proteiniclasticum</i>. Redundancy analysis showed gut microbial composition as the primary driver of MRG abundance, with MGEs, tilmicosin concentration, and manure physicochemical properties playing secondary roles. These findings suggest that high-dose tilmicosin may alter the gut microbiota and promote ARG spread via MGE-mediated transfer. |
| format | Article |
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| institution | Kabale University |
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| publishDate | 2024-12-01 |
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| spelling | doaj-art-4d4b8820695a41ad95ce6bbf3f3a7de72025-01-10T13:13:58ZengMDPI AGAnimals2076-26152024-12-011517010.3390/ani15010070High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic ElementsTao Chen0Minxing Zhao1Majian Chen2Xiaoyue Tang3Yuliang Qian4Xiaoting Li5Yan Wang6Xindi Liao7Yinbao Wu8College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaPhage Research Center, Liaocheng University, Liaocheng 252000, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaThe impact of antibiotic therapy on the spread of antibiotic resistance genes (ARGs) and its relationship to gut microbiota remains unclear. This study investigated changes in ARGs, mobile genetic elements (MGEs), and gut microbial composition following tilmicosin administration in pigs. Thirty pigs were randomly divided into control (CK), low-concentration (0.2 g/kg; L), and high-concentration (0.4 g/kg; H) groups. Tilmicosin concentration in manure peaked on day 16 of dosing and dropped below detectable levels by day 13 of the withdrawal period. While tilmicosin did not significantly affect the total abundance of macrolide resistance genes (MRGs) (<i>p</i> > 0.05), it significantly increased the abundance of the multidrug resistance gene <i>tol</i>C in the H group compared with the L and CK groups during the withdrawal period (<i>p</i> < 0.05). This increase was associated with a coincidental rise in the abundance of MGEs (e.g., <i>int</i>1 and <i>int</i>2) and the growth of potential <i>tol</i>C-hosting bacteria such as <i>Paenalcaligenes</i> and <i>Proteiniclasticum</i>. Redundancy analysis showed gut microbial composition as the primary driver of MRG abundance, with MGEs, tilmicosin concentration, and manure physicochemical properties playing secondary roles. These findings suggest that high-dose tilmicosin may alter the gut microbiota and promote ARG spread via MGE-mediated transfer.https://www.mdpi.com/2076-2615/15/1/70tilmicosingut microbiomeantibiotic resistance genesmobile genetic elementsantibiotic excretion |
| spellingShingle | Tao Chen Minxing Zhao Majian Chen Xiaoyue Tang Yuliang Qian Xiaoting Li Yan Wang Xindi Liao Yinbao Wu High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic Elements Animals tilmicosin gut microbiome antibiotic resistance genes mobile genetic elements antibiotic excretion |
| title | High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic Elements |
| title_full | High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic Elements |
| title_fullStr | High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic Elements |
| title_full_unstemmed | High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic Elements |
| title_short | High Concentrations of Tilmicosin Promote the Spread of Multidrug Resistance Gene <i>tol</i>C in the Pig Gut Microbiome Through Mobile Genetic Elements |
| title_sort | high concentrations of tilmicosin promote the spread of multidrug resistance gene i tol i c in the pig gut microbiome through mobile genetic elements |
| topic | tilmicosin gut microbiome antibiotic resistance genes mobile genetic elements antibiotic excretion |
| url | https://www.mdpi.com/2076-2615/15/1/70 |
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