Barcode-free multiplex plasmid sequencing using Bayesian analysis and nanopore sequencing

Plasmid construction is central to life science research, and sequence verification is arguably its costliest step. Long-read sequencing has emerged as a competitor to Sanger sequencing, with the principal benefit that whole plasmids can be sequenced in a single run. Nevertheless, the current cost o...

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Bibliographic Details
Main Authors: Masaaki Uematsu, Jeremy M Baskin
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-04-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/88794
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