Simple Strategy for Sequencing cDNA Clones
We describe a simple method for constructing subclones containing overlapping nested deletions from cDNA clones (both λ phage clones and plasmid clones). A PCRamplified insert is partially digested with 4-cutter restriction enzyme(s). Complete digestion of this DNA with two restriction enzymes, havi...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96213st01 |
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