First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh
Abstract Enterobacter asburiae (E. asburiae) is a gram-negative rod-shaped bacterium which has emerging significance as an opportunistic pathogen having high virulence pattern and drug resistant properties. In this study, we present the detailed analysis of the whole genome sequence of a multidrug-r...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84870-7 |
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| author | Md. Rafiul Islam Spencer Mark Mondol Md. Azad Hossen Mst. Poli Khatun Shahjada Selim Amiruzzaman Donald James Gomes Md. Mizanur Rahaman |
| author_facet | Md. Rafiul Islam Spencer Mark Mondol Md. Azad Hossen Mst. Poli Khatun Shahjada Selim Amiruzzaman Donald James Gomes Md. Mizanur Rahaman |
| author_sort | Md. Rafiul Islam |
| collection | DOAJ |
| description | Abstract Enterobacter asburiae (E. asburiae) is a gram-negative rod-shaped bacterium which has emerging significance as an opportunistic pathogen having high virulence pattern and drug resistant properties. In this study, we present the detailed analysis of the whole genome sequence of a multidrug-resistant (MDR) E. asburiae strain BDW1M3 from Bangladesh. The isolate was collected from an infected foot wound of a diabetic foot ulcer patient. Through sophisticated genomic techniques encompassing whole genome sequencing and in-depth bioinformatic analyses, this research unveils a profound understanding of the isolate’s antimicrobial resistance patterns, virulence determinants, biosynthetic gene clusters, metabolic pathways and pathogenic potential. The isolate displayed resistance to Ampicillin, Fosfomycin, Cefoxitin, Tigecycline, Meropenem, Linezolid, Vancomycin antibiotics and demonstrated the capacity for biofilm formation. Several antimicrobial resistance genes such as bla ACT−2, fosA2, baeR, qnrE2, vanA and numbers of virulence genes including ybaJ, csrA, barA, uvrY, pgaD, hlyD, hlyC, terC, purD were detected. Metal resistance genes investigation revealed the presence of cusCFBA operon system, and many other genes including zntA, zitB, czrB. Prophage region of Myoviridae was detected. Comparative genomics with 47 whole genome sequence (n = 47) shed light on the genetic diversity of E. asburiae strains from diverse sources and countries, with a notable observation that strains from both human and non-human origins exhibited significant pathogenicity potential, genomic and phylogenomic relations hinting at potential cross-species transmission. Pangenome analysis indicated toward an expanding pangenome of E. asburiae. Further research and in-depth comprehensive studies are required to investigate the prevalence of E. asburiae in Bangladesh and emphasize towards unraveling the bacterium’s inherent pathogenic potential and the intricate molecular mechanisms that underlie its resistance traits and virulence properties. |
| format | Article |
| id | doaj-art-4c694825e80f42078e82ca2260c35259 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4c694825e80f42078e82ca2260c352592025-01-05T12:18:48ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-024-84870-7First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from BangladeshMd. Rafiul Islam0Spencer Mark Mondol1Md. Azad Hossen2Mst. Poli Khatun3Shahjada Selim4Amiruzzaman5Donald James Gomes6Md. Mizanur Rahaman7Department of Microbiology, University of DhakaDepartment of Microbiology, University of DhakaDepartment of Microbiology, University of DhakaDepartment of Microbiology, University of DhakaDepartment of Endocrinology, Bangabandhu Sheikh Mujib Medical UniversityDepartment of Medicine, Sir Salimullah Medical CollegeDepartment of Microbiology, University of DhakaDepartment of Microbiology, University of DhakaAbstract Enterobacter asburiae (E. asburiae) is a gram-negative rod-shaped bacterium which has emerging significance as an opportunistic pathogen having high virulence pattern and drug resistant properties. In this study, we present the detailed analysis of the whole genome sequence of a multidrug-resistant (MDR) E. asburiae strain BDW1M3 from Bangladesh. The isolate was collected from an infected foot wound of a diabetic foot ulcer patient. Through sophisticated genomic techniques encompassing whole genome sequencing and in-depth bioinformatic analyses, this research unveils a profound understanding of the isolate’s antimicrobial resistance patterns, virulence determinants, biosynthetic gene clusters, metabolic pathways and pathogenic potential. The isolate displayed resistance to Ampicillin, Fosfomycin, Cefoxitin, Tigecycline, Meropenem, Linezolid, Vancomycin antibiotics and demonstrated the capacity for biofilm formation. Several antimicrobial resistance genes such as bla ACT−2, fosA2, baeR, qnrE2, vanA and numbers of virulence genes including ybaJ, csrA, barA, uvrY, pgaD, hlyD, hlyC, terC, purD were detected. Metal resistance genes investigation revealed the presence of cusCFBA operon system, and many other genes including zntA, zitB, czrB. Prophage region of Myoviridae was detected. Comparative genomics with 47 whole genome sequence (n = 47) shed light on the genetic diversity of E. asburiae strains from diverse sources and countries, with a notable observation that strains from both human and non-human origins exhibited significant pathogenicity potential, genomic and phylogenomic relations hinting at potential cross-species transmission. Pangenome analysis indicated toward an expanding pangenome of E. asburiae. Further research and in-depth comprehensive studies are required to investigate the prevalence of E. asburiae in Bangladesh and emphasize towards unraveling the bacterium’s inherent pathogenic potential and the intricate molecular mechanisms that underlie its resistance traits and virulence properties.https://doi.org/10.1038/s41598-024-84870-7Enterobacter asburiaeMultidrug resistantDiabetic foot infectionDiabetic foot ulcerWhole genome sequencingBangladesh |
| spellingShingle | Md. Rafiul Islam Spencer Mark Mondol Md. Azad Hossen Mst. Poli Khatun Shahjada Selim Amiruzzaman Donald James Gomes Md. Mizanur Rahaman First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh Scientific Reports Enterobacter asburiae Multidrug resistant Diabetic foot infection Diabetic foot ulcer Whole genome sequencing Bangladesh |
| title | First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh |
| title_full | First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh |
| title_fullStr | First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh |
| title_full_unstemmed | First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh |
| title_short | First report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh |
| title_sort | first report on comprehensive genomic analysis of a multidrug resistant enterobacter asburiae isolated from diabetic foot infection from bangladesh |
| topic | Enterobacter asburiae Multidrug resistant Diabetic foot infection Diabetic foot ulcer Whole genome sequencing Bangladesh |
| url | https://doi.org/10.1038/s41598-024-84870-7 |
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