Engineering glycosyltransferases into glycan binding proteins using a mammalian surface display platform

Abstract Traditional lectins exhibit broad binding specificity for cell-surface carbohydrates, and generating anti-glycan antibodies is challenging due to low immunogenicity. Nevertheless, it is necessary to develop glycan binding proteins for single-cell glycosylation pathway analysis. Here, we tes...

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Bibliographic Details
Main Authors: Ryoma Hombu, Lauren E. Beatty, John Tomaszewski, Sheldon Park, Sriram Neelamegham
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-62018-z
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